Clara细胞分泌蛋白10基因G38A多态性与5岁以下喘息患儿的相关性

目的探讨5岁以下喘息患儿Clara细胞分泌蛋白10(CC10)基因G38A位点多态性与喘息发病机制的关系。方法随机选取于本院就诊的5岁以下反复喘息患儿120例,分为有特应质高危因素的喘息Ⅰ组(n=67)(湿疹45例,父母或父母一方有哮喘病史13例,变应性鼻炎5例,变应性皮炎4例)和无特应质高危因素的喘息Ⅱ组(n=53);对照组为本院外科近期无感染疾病史、择期进行手术的术前患儿(n=55)。采用PCR-限制性片段长度多态性分析对喘息组和对照组患儿CC10 G38A位点基因型频率和等位基因频率进行检测,比较3组间CC10 G38A位点基因型频率和等位基因频率。结果喘息Ⅰ组、喘息Ⅱ组和对照组3种基因型AA、GA、GG分布频率分别为20.9%、44.8%、34.3%,9.4%、32.1%、58.5%、9.1%、31.0%、60.0%;喘息Ⅰ组和喘息Ⅱ组CC10基因G38A位点基因型频率比较差异有统计学意义(P<0.05);喘息Ⅰ组和对照组CC10基因G38A位点基因型频率比较差异亦具有统计学意义(P<0.05)。喘息Ⅰ组、喘息Ⅱ组和对照组38A和38G等位基因频率分别为43.3%、56.7%,25.5%、74.5%,24.5%、75.5%;喘息Ⅰ组和喘息Ⅱ组、喘息Ⅰ组和对照组比较差异均有统计学意义(Pa<0.05)。结论喘息患儿与哮喘存在相同的基因分布频率,发生哮喘的危险性高;对于CC10基因具有A等位基因的喘息患儿应密切关注。

Relationship between Clara Cell Protein 10 Gene G38A Polymorphism and Pathogens of Wheezing in Children under 5 Years Old

Objective To study the relationship between Clara cell protein 10(CC10) gene G38A polymorphism and the pathogens of wheezing in children under 5 years old. Methods Children under 5 years old with recurrent wheezing had been randomly enrolled.They were divided into 2 groups:wheezing groupⅠwith atopic high risks included 67 cases(including eczema 45 cases,or either of their parents had a history of asthma risk factors for atopic quality standards 13 cases,atopic rhinitis 5 cases,atopic dermatitis 4 cases);and wheezing groupⅡwithout atopic high risks(n=53).Children with surgical operation selectly,without recent infectious diseases had been enrolled randomly as control group(n=55).Polymerase chain reaction restriction enzyme cutting technology was used to detect CC10 gene G38A allele frequency and genotypic frequency. Results Frequencies of AA,GA and GG genotypes in wheezing groupⅠ,wheezing groupⅡ and control group were 20.9%,44.8%,34.3%,9.4%,32.1%,58.5%,9.1%,31.0%,60.0%;there were significant differences in A38,G38 allele frequency between wheezing groupⅠand wheezing groupⅡ,wheezing groupⅠand control group(Pa<0.05).Frequencies of A,G allele were 43.3%,56.7%,25.5%,74.5%,24.5%,75.5% in wheezing groupⅠ,wheezing groupⅡand control group.There were significant differences in A38,G38 allele genotypic frequency in wheezing groupⅠand wheezing groupⅡ,wheezing groupⅠand control group(Pa<0.05). Conclusions Wheezing children have the same gene frequency to asthmatic children,which the risk of asthma occurrence is high,we should pay more attention to wheezing children with CC10 gene,allele A genotypic.