| ELISA法测定甲型H1N1流感病毒裂解疫苗中卵清蛋白含量的不确定度分析 |
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| 引用本文： | 王灿,陆明,吴利红,邵泓,陈钢.ELISA法测定甲型H1N1流感病毒裂解疫苗中卵清蛋白含量的不确定度分析[J].药物分析杂志,2012(4):676-678. |
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| 作者姓名： | 王灿 陆明 吴利红 邵泓 陈钢 |
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| 作者单位： | 上海市食品药品检验所 |
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| 摘 要： | 目的:对ELISA法测定甲型H1N1流感病毒裂解疫苗中卵清蛋白含量不确定度进行评定分析。方法:按照卵清蛋白检测试剂盒说明书对甲型H1N1流感病毒裂解疫苗中卵清蛋白含量进行测定。根据《测量不确定度评定与表示》(JJF1059-1999)中有关规定对测量结果中带来的各个不确定度进行分析、评定和量化。结果:量化各个不确定度分量,提出了该法的合成不确定度评估结果。结论:该法测定甲型H1N1流感病毒裂解疫苗中卵清蛋白含量的扩展不确定度U=0.68 ng.mL-1(k=2)。
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| 关 键 词： | 酶联免疫吸附试验 甲型H1N1流感病毒裂解疫苗 卵清蛋白含量 不确定度 |
Analysis of uncertainty measurement in determination of ovalbumin with ELISA |
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| WANG Can,LU Ming,WU Li-hong,SHAO Hong,CHEN Gang.Analysis of uncertainty measurement in determination of ovalbumin with ELISA[J].Chinese Journal of Pharmaceutical Analysis,2012(4):676-678. |
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| Authors: | WANG Can LU Ming WU Li-hong SHAO Hong CHEN Gang |
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| Institution: | (Shanghai Institute for Food and Drug Control,Shanghai 201203,China) |
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| Abstract: | Objective:To analyze the measurement uncertainty in determination of ovalbumin with ELASA.Methods:The assay of ovalbumin was detected in accordance with the instruction of ovalbumin detection kit,and the measurement uncertainty was evaluated according to JJF1059-1999.Results:The expanded measurement uncertainties were quantified,and the evaluation results of measurement uncertainty was drawn.Conclusion:The expanded uncertainty was U=0.68 ng·mL-1(k=2). |
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| Keywords: | ELISA H1N1 influenza virus split vaccine ovalbumin assay uncertainty analysis |
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