| 复合型完全脱蛋白骨的细胞相容性研究 |
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| 引用本文： | 李彦林,杨志明,韩睿,李世和,曾才铭. 复合型完全脱蛋白骨的细胞相容性研究[J]. 中国骨伤, 2002, 15(10): 597-599 |
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| 作者姓名： | 李彦林 杨志明 韩睿 李世和 曾才铭 |
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| 作者单位： | 1. 昆明医学院第一附属医院骨科,云南,昆明,650032
2. 华西医科大学附一院骨科,四川,成都 |
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| 基金项目： | 云南省自然科学基金资助项目(2000C0010R) |
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| 摘 要： | 目的 探讨复合型完全脱蛋白骨的细胞相容性。方法 将猪肋骨用理化方法制得复合型完全脱蛋白骨（CFDB），对CFDB理化性能进行检测，又将其与人胎骨膜成骨细胞体外复合培养，以了解其细胞相容性及细胞毒性，结果 CFDB的主要成份为羟基磷灰石，蛋白质含量几乎为零，仍具有原骨组织骨盐支架的三维多孔网状孔隙结构系统，对复合培养的人胎骨膜成骨细胞的形态特征，ALP活性，细胞周期，倍体水平无有害影响。结论 CFDB具有原骨组织的网状孔隙结构系统，具有良好的细胞相容性，可望用于成骨细胞的支架材料。
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| 关 键 词： | 成骨细胞 细胞培养 复合型完全脱蛋白骨 |
| 收稿时间： | 2001-11-26 |
| 修稿时间： | 2002-03-15 |
Cellular compatibility study of composite fully deproteinised bone |
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| LI Yan-lin,YANG Zhi-ming,HAN Rui. Cellular compatibility study of composite fully deproteinised bone[J]. China journal of orthopaedics and traumatology, 2002, 15(10): 597-599 |
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| Authors: | LI Yan-lin YANG Zhi-ming HAN Rui |
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| Affiliation: | The First Affiliated Hospital of Kunming Medical College Yunnan Kunming,650032;The First Affiliated Hospital of Kunming Medical College Yunnan Kunming,650032;The First Affiliated Hospital of Kunming Medical College Yunnan Kunming,650032;The First Affiliated Hospital of Kunming Medical College Yunnan Kunming,650032 |
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| Abstract: | Objective:To study the cellular compatibility of composite fully deproteinised bone(CFDB) Methods:CFDB was processed by physical and chemical methods with pig ribs and its properties were examined, Then its cellular compatibility and cytotoxicity were examined by osteoblasts culture in vitro. Results:The main ingredients of CFDB was hydroxyapatite;it hardly had any protein,and it had natural pore system which possessed pore network system of original bone. It has no bad affect on cell feature,alkaline phosphatase activity and cell cycle of human embryonic periosteal-derived osteoblasts composited with CFDB. Conclusion:CFDB possessed original bone natural pore system and well cellular compatibility,and it could be used as scalfolds of osteoblasts. |
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| Keywords: | Osteoblast Cell culture Composite fully deproteinised bone |
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