miR-203a-3p在胰腺癌组织与细胞中的表达及其对BxPC-3细胞增殖、迁移和侵袭的影响

目的:探讨miR-203a-3p对胰腺癌BxPC-3细胞增殖、迁移和侵袭能力的影响.方法:运用癌症基因组图谱(TCGA)数据库筛选胰腺癌组织和癌旁组织中差异表达的miRNA,分析miRNA高表达与低表达时胰腺癌患者的生存率和临床分期;利用TarBase数据库分析miRNA与癌症相关的GO功能与KEGG通路,利用DIAN...

Expression of miR-203a-3p in pancreatic cancer tissues and cells and its effect on proliferation, migration and invasion of BxPC-3 cells

Objective: To investigate the effects of miR-203a-3p on the proliferation, migration and invasion ability of pancreatic cancer BxPC-3 cells. Methods: The Cancer Genome Atlas (TCGA) database was used to screen the differentially expressed miRNAs between pancreatic cancer tissues and paracancerous tissues, and to analyze the survival rate and clinical stage of pancreatic cancer patients with high or low miRNA expression; TarBase was used for the cancer related GO function and KEGG pathway analysis of the miRNAs, DIANATools, miRDB and TargetScan websites were used to predict the target genes of miR-203a-3p. miR-203a-3p mimic and NC mimic, miR-203a-3p inhibitor and NC inhibitor were transfected into BxPC-3 cells. The expression levels of miR-203a-3p, miR-192-5p and miR-451a in normal pancreatic epithelial HPNE cells and pancreatic cancer cells were detected by qPCR. The proliferation, migration, invasion and colony formation abilities of BxPC-3 cells were detected by CCK-8, Transwell chamber assay and Colony formation assay, respectively. Results: A total of 18 differentially expressed miRNAs in pancreatic cancer tissues were screened out by TCGA database, among which miR-203a[1]3p, miR-192-5p and miR-451a were species-conservative and significantly correlated with clinical cancer stage, cell cycle and survival rate of pancreatic cancer patients (all P<0.05); Bioinformatics tool predicted that PPM1A might be the candidate target gene of miR[1] 203a-3p and could interact with multiple genes. miR-203a-3p, miR-192-5p and miR-451a were highly expressed in BxPC-3 and Aspc[1] cells (all P<0.001). In miR-203a-3p mimic group, the expression level of miR-203a-3p and the proliferation, migration and invasion ability of BxPC-3 cells were significantly increased (all P<0.01); however, in miR-203a-3p inhibitor group, the expression level of miR-203a-3p and the ability of cell proliferation, migration and invasion were significantly decreased (all P<0.01). Conclusion: miR-203a-3p is highly expressed in pancreatic cancer tissues and cells, and its expression is related to the survival and clinical stage of patients. miR-203a-3p may regulate the proliferation, migration and invasion of BxPC-3 cells.