手足口病患儿外周血细胞因子及T淋巴细胞检测结果分析

目的检测手足口病患儿外周血细胞因子和免疫T淋巴细胞变化，并与正常儿童对比，探讨其免疫学发病机制。方法细胞因子（白介素6、10、17、21、23）检测采用酶联免疫吸附（ELISA）法，免疫T淋巴细胞（CD3＋、CD3＋CD4＋、Treg细胞、Thl7细胞）检测采用流式细胞仪。结果①手足口病患儿白介素6、10、17、21、23结果均升高，分别是（40±0．8）、（54±1．1）、（33±0．6）、（33±0．9）和（27±0．8）pg／L；正常小儿对照组分别为（17±1．0）、（23±0．6）、（19±0．8）、（21±0．6）和（19±0．8）pg／L，两组比较，差异有统计学意义（P〈0．05）。②免疫T淋巴细胞CD3＋、CD3＋CD4＋、Treg细胞、Th17细胞分别为（44±0．9）％、（27±0．7）％、（20±0．4）％、（28±1．0）％；正常小儿对照组分别为（52±1．1）％、（44±1．1）％、（24±O．7）％、（3．6±0．5）％，Th17细胞升高，两组比较，差异有统计学意义（P〈0．05）。结论手足口病患儿急性期白介素升高，Th17细胞升高，存在免疫系统功能紊乱。

Test of serum cytokines and lymphocytes in children with hand, foot-and mouth disease

Objective ] To investigated the changes and significances of cyterkines and lymphocytes in children with hand, foot and mouth disease（HFMD）. Methods ] Fifty nine cases with HFMD and thirty healthy children as controls were enrolled in study. The percentage of lymphocyte subsets（including CD3＋, CD3＋CD4＋, Treg and Thl7 cells） and cyterkines（including interleukin 6,10,17,21,23） in two groups were determined by four color fluorescence flow cytometry and ELISA. Resluts ] Compared with normal controls, the HFMD patients showed an obvious decrease in percentage of CD3＋, CD3＋ CD4＋,Treg cells, but obvious elevation of Thl7cells and cyterkines. Significant difference was found in two groups. Conclusion ] There are functional disorders in cellular and humoral immunity in HFMD patients, Abnormal immunity may play an important role in the children with HFMD.