高效液相色谱法同时测定田基黄药材中槲皮素、芦丁和异鼠李素的含量

目的建立高效液相色谱法同时测定田基黄药材中槲皮素、芦丁和异鼠李素的含量。方法采用HPLC法，色谱柱为Agilct Hypcrsil C18（4．6mm×250mm，5μm），甲醇-0．2％磷酸（54：46）为流动相，流速为1mL·min^-1，检测波长264mm，柱温为室温。结果槲皮素、芦丁和异鼠李素分别在0．265-4．240、0．201～3．22、0.0755～1．208μg线性关系良好，平均回收率分别为99．7％0、99．7％、100．80，RSD分别为0．76%、1.28％、2．69％。结论所建立的方法简单易行，准确可靠，可用于田基黄药材中槲皮素、芦丁和异鼠李素的含量测定。

Simultaneous determination of quercetin,rutoside and isorhamnetin in Hypericum japonicum Thunb. by HPLC

Objective To establish an HPLC method for simultaneous content determination of quercetin, rutoside and isorhamnetin in Hypericurnjaponicum Thunb.. Methods HPLC method was used. Agilet Hypersil C18 column （4. 6 mm×250 mm, 5 μm） was used with methanol-0.2%phosphoric acid （54 ： 46） as the mobile phase. The flow rate Was 1 mL · min^- 1. The detection wavelength was 261 nm, and the column temperature was room temperature. Results The linear range of quercetin, rutoside and isorhamnetin was 0. 265-4. 240, 0. 201 3.22, and 0. 0755- 1. 208 ptg. The average recovery was 99.7%, 99.7% and 100.8% with RSD was 0.76%, 1.28% and 2.69%, respectively. Conclusion The method is sensitive, accurate and can be used for content of quercetin, rutoside and isorhamnetin in Hypericum japonicum Thunb.