| 环孢素A对兔晶状体上皮细胞增殖过程中磷酸肌醇-3激酶途径的影响 |
| |
| 引用本文： | 赵宁,张瑞君,钟一凡,刘磊,李佳.环孢素A对兔晶状体上皮细胞增殖过程中磷酸肌醇-3激酶途径的影响[J].国际眼科杂志,2014,14(12):2135-2138. |
| |
| 作者姓名： | 赵宁 张瑞君 钟一凡 刘磊 李佳 |
| |
| 作者单位： | 中国医科大学附属第一医院眼科, 中国辽宁省沈阳市,110001 |
| |
| 基金项目： | 中国辽宁省自然科学基金项目(No.201102259) |
| |
| 摘 要： | 目的:研究环孢素A对白内障术后晶状体上皮细胞增殖过程中磷酸肌醇-3激酶(phosphatidylinositol-3 kinase,PI-3 k)途径的影响,为后发性白内障的治疗提供实验依据。方法:将健康白色家兔30只60眼,行双眼透明晶状体皮质吸除术,右眼为治疗组,左眼为对照组。术后第1d起,对照组眼用生理盐水点眼6次,而治疗组眼应用1%环孢素A(cyclosporine A,Cs A)眼药水点眼6次。分别在术后第1d未点药前、术后1wk,2wk,1mo和2mo各处死随机选择的6只兔,摘取双眼球。应用免疫组织化学、原位杂交方法检测赤道部晶状体上皮细胞中增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)及张力蛋白同源的28号染色体缺失磷酸酯酶mRNA(PTEN mRNA)和Ser473-R的表达。结果:术后两组PCNA和Ser473-R的表达均逐渐降低,其中1wk(0.690±0.035 vs 0.785±0.015,t=6.099,P<0.01)和2wk(0.571±0.038 vs 0.670±0.037,t=4.585,P<0.01)时治疗组PCNA表达均显著低于对照组。另外,1wk(0.374±0.031 vs 0.435±0.030,t=3.486,P=0.006)和2wk(0.220±0.022 vs 0.251±0.020,t=2.516,P=0.031)时治疗组Ser473-R的表达均显著低于对照组。术后1wk~1mo时,两组PTEN mRNA均逐渐回升。术后1wk,治疗组A值显著高于对照组(0.302±0.027 vs 0.255±0.038,t=2.474,P=0.033)。结论:环孢素A对兔眼白内障术后晶状体上皮细胞增殖过程中PI-3k途径有显著抑制作用,为研究CsA抑制后发性白内障提供实验依据。
|
| 关 键 词： | 环孢素A 晶状体上皮细胞 后发性白内障 PTEN |
| 收稿时间： | 8/6/2014 12:00:00 AM |
| 修稿时间： | 2014/11/20 0:00:00 |
Effect of cyclosporine A on the PI-3k pathway in proliferation of rat lens epithelial cells |
| |
| Ning Zhao,Rui-Jun Zhang,Yi-Fan Zhong,Lei Liu and Jia Li.Effect of cyclosporine A on the PI-3k pathway in proliferation of rat lens epithelial cells[J].International Journal of Ophthalmology,2014,14(12):2135-2138. |
| |
| Authors: | Ning Zhao Rui-Jun Zhang Yi-Fan Zhong Lei Liu and Jia Li |
| |
| Institution: | Department of Ophthalmology, the First Hospital of China Medical University, Shenyang 110001, Liaoning Province, China;Department of Ophthalmology, the First Hospital of China Medical University, Shenyang 110001, Liaoning Province, China;Department of Ophthalmology, the First Hospital of China Medical University, Shenyang 110001, Liaoning Province, China;Department of Ophthalmology, the First Hospital of China Medical University, Shenyang 110001, Liaoning Province, China;Department of Ophthalmology, the First Hospital of China Medical University, Shenyang 110001, Liaoning Province, China |
| |
| Abstract: | |
| |
| Keywords: | cyclosporine A lens epithelial cells after cataract gene of phosphate and tension homology deleted on chromsome ten |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《国际眼科杂志》浏览原始摘要信息 |
| 点击此处可从《国际眼科杂志》下载免费的PDF全文 |
|
