|
|||||
|
|
| 大鼠MSCs体外分离培养及骨向分化的实验研究 | |
| 引用本文： | 熊曦州,李晓红,姚天华,饶国洲,石建峰,李昂. 大鼠MSCs体外分离培养及骨向分化的实验研究[J]. 中国美容医学, 2006, 15(4): 379-381,i0002 |
| 作者姓名： | 熊曦州 李晓红 姚天华 饶国洲 石建峰 李昂 |
| 作者单位： | 1. 西安市第二医院·西安市口腔医院 2. 西安交通大学口腔种植中心 3. 西安交通大学口腔医学研究中心 |
| 基金项目： | 西安交通大学附属口腔医院青年科学基金 |
| 摘 要： | 目的:建立骨髓MSCs体外分离培养体系,并进行骨向分化诱导,以证实其多向分化潜能,为骨髓MSCs进一步的临床应用研究提供实验依据。方法:用密度梯度离心法分离大鼠骨髓MSCs,并对其形态学特征进行观察。用诱导剂对骨髓MSCs向成骨细胞进行诱导分化,并进行形态学观察和免疫细胞化学检测。结果:用密度梯度离心法成功分离获得了高纯度的骨髓MSCs。经骨向诱导后,ALP和矿化结节染色阳性。结论:采用密度梯度离心法成功建立了大鼠骨髓MSCs体外分离和培养体系,并能够向成骨细胞分化。 |
| 关 键 词： | 骨髓间充质干细胞 细胞培养 骨向分化 |
| 文章编号： | 1008-6455（2006）04-0379-02 |
| 收稿时间： | 2005-09-22 |
| 修稿时间： | 2006-01-16 |
An Experimental Study of Isolation,Culture and Osteogenic Differentiation of Rat Bone Marrow Stem Cell in Vitro |
|
| XIONG Xi-zhou,LI Xiao-hong,YAO Tian-hua,RAO Guo-zhou,SHI Jian-feng,LI Ang. An Experimental Study of Isolation,Culture and Osteogenic Differentiation of Rat Bone Marrow Stem Cell in Vitro[J]. Chinese Journal of Aesthetic Medicine, 2006, 15(4): 379-381,i0002 | |
| Authors: | XIONG Xi-zhou LI Xiao-hong YAO Tian-hua RAO Guo-zhou SHI Jian-feng LI Ang |
| Abstract: | Objective To establish an isolation and culture system of rat bone marrow stem cells and Osteogenic differentiation in vitro in order to verify their pluripotential.Methods Density gradient centrifugation was used to isolate bone marrow stem cells from SD rats.The BMSCs were also undergone osteogenic induction and cytochemical and immunocytochemical staining were performed to verify their multipotential.Results High purity of bone marrow stem cells was successfully obtained.Both ALP activity and mineraliztion node staining were positive in BMSCs after osteogenic differentiation.Conclusion The isolation and culture system of rat bone marrow stem cells was successfully established and the BMSCs sustained their osteogenic differentiation potential in vitro. |
| Keywords: | bone marrow stem cells cell culture osteogenic differentiation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |