广西田七总黄酮的超声波提取工艺及含量测定

目的：以总黄酮为指标，优化超声波提取田七黄酮的最佳工艺条件，测定广西田七不同部位黄酮含量。方法：运用正交设计筛选超声提取参数，以芦丁为对照品，采用NaNO2-Al（NO3）3-NaOH比色法测定总黄酮含量。结果：采用φ（C2H6O）=70％为溶剂，预先浸泡2h，超声波提取3次，每次40min，以固液比为1：20为最佳超声提取工艺。并测定田七中不同部位黄酮含量，分别为根茎1．394mg／g，主根0．646mg／g，侧根0．785mg／g，须根0．982mg／g。结论：超声波提取工艺简便，方法可行；不同部位黄酮含量差异较大，根茎含量最高，侧根和须根其次，而主根含量最低。

Extraction Technique by Ultrasonic Wave and Content Determination of the Total Flavone from TianQi in Guangxi

Objective:To establish the optimized process of the total flavone extraction in Panax notoginseng (Tianqi) by ultrasonic wave technique,and to determinate the total flavone content.Methods:The extraction conditions with ultrasonic wave were studied by orthogonal design and the content of flavone was determined by the NaNO_2- Al(NO_3)_3-NaOH colorimetric method.Results:The optimique process of the technique was:the ratio ofS]/L]1:20, the extraction time 40 min and extracted 3 times withφ(C_2H_6O)=70%.The flavone content of rhizome,taproot, secondary root and fibre root was 1.394 mg/g,0.646 mg/g,0.785 mg/g and 0.982 mg/g respectively.Conclusion:the ultrasonic wave extraction technique of flavone is convenient and the content of the total flavone in Panax notoginseng in Guangxi province was different in different parts:rhizome was the highest and taproot was the lowest.