冠心胶囊对大鼠心肌缺血的保护作用

 目的 观察冠心胶囊对大鼠心肌梗死的影响及其作用机制。方法 用大鼠冠脉结扎法制备心肌梗死模型，应用膜片钳技术研究药物对L-型钙通道的作用。结果 冠心胶囊能剂量依赖性地对抗大鼠冠脉结扎引起的心肌梗死，并明显抑制心肌缺血引起的S-T段抬高。冠心胶囊1，10，30 mg·ml^-1可显著抑制钙通道，减少钙离子内流，30 mg·ml^-1可抑制钙离子52%(P＜0.01)。结论 冠心胶囊能剂量依赖性地缩小大鼠心肌梗死面积，对心梗具有保护作用。这一作用可能和其调节植物神经的功能，抑制钙通道，稳定心肌细胞膜有关。

Protect effect of Guanxin capsule on myocardial ischemia in rats

OBJECTIVE To observe the effect and mechanism of Guanxin capsule on myocardial infarction in rats. METHODS The myocardial infarction model in rats was induced by coronary artery ligation.The effect of Guanxin capsule on L-type calcium channel current was studied by using patch-clamp whole cell recording techniques. RESULTS Guangxin capsule showed marked effects on myocardial infarction in a dose-dependent fashion,and significantly inhibited the elevation of S-T segment induced by myocardial ischemia.In patch-clamp experiment,Guanxin 1，10，30 mg·ml^-1specifically inhibited L-type calcium current in a concentration-dependent fashion,30 mg·ml^-1inhibited calcium current by 52%. CONCLUSION Guanxin capsule shrinked the myocardial infarction area in rats in a dose-dependent fashion,and had the protect effect on myocardial infarction.The mechanism of Guanxin capsule on myocardial infarction and ischemia might be related to its regulating nerve function,inhibiting calcium channel,and stabilizing cardiac myocyte membrane.