盐酸多奈哌齐对血管性痴呆小鼠认知功能及P300电位的影响

目的探讨具有认知功能改变的血管性痴呆小鼠P300电位的变化特征及盐酸多奈哌齐对其影响。方法48只小鼠随机分为假手术组、血管性痴呆模型组(模型组)和多奈哌齐组,每组16只。应用双侧颈总动脉线结反复缺血再灌注法制备血管性痴呆小鼠模型,采用盐酸多奈哌齐连续治疗4周,利用跳台试验和水迷宫试验观测其行为学改变,测定P300电位。结果与多奈哌齐组和假手术组比较,模型组小鼠学习成绩为反应时间长,游全程时间长,错误次数多,记忆成绩为跳台试验潜伏时间短,游全程时间长,错误次数多(P<0.05)。模型组小鼠P300电位的测定,N2潜伏期、P3潜伏期均长于多奈哌齐组和假手术组(P<0.01);P3波幅低于多奈哌齐组和假手术组(P<0.05)。结论盐酸多奈哌齐对血管性痴呆认知功能损伤有肯定的干预和治疗作用。

Effect of donepezil hydrochloride on cognitive function and P300 potential of mice with vascular dementia

Objective To explore the feature of changes of event-related potential P300 and the effects of donepezil hydrochloride in vascular dementia mice with cognitive function change.Methods Forty-eight mice were randomized to sham operation group,vascular dementia model group and treatment with donepezil hydrochloride group.Each group included 16 mice.The mice were subjected to ischemia and reperfusion repeatedly by the ligation of bilateral common carotid arteries to establish the VaD models.They were treated with donepezil hydrochloride for 4 weeks.The behavioral changes were investigated by step-down test and water maze test.N2 and P3 were determined in mice with vascular dementia treated with donepezil hydrochloride and compared with those in the sham operation group and VaD model group.Results Compared with the sham operation group and mice with vascular dementia treated with donepezil hydrochloride,the learning records of model group mice were that the response time was longer,swimming time was longer and the error frequency was increased.Memory records of model group mice by step-down test were that latent time was shorter and in water maze test,the swimming time was longer and the error frequency was increased(P<0.05).N2 and P3 potential latencies of model group mice were significantly longer than those of VaD mice treated with donepezil hydrochloride and sham operation group mice(P<0.01);P3 amplitude was lower than that of the VaD mice treated with donepezil hydrochloride and sham operation group mice(P<0.05).Conclusion Donepezil hydrochloride can intervene and treat damage of the cognitive functions in mice with vascular dementia