| 3个鼠源性肿瘤线粒体DNA编码区基因RFLP分析 |
| |
| 引用本文： | 戴纪刚,魏泓,闵家新,张国强.3个鼠源性肿瘤线粒体DNA编码区基因RFLP分析[J].重庆医学,2004,33(11):1674-1675,1678. |
| |
| 作者姓名： | 戴纪刚 魏泓 闵家新 张国强 |
| |
| 作者单位： | 第三军医大学新桥医院胸外科,重庆,400037;第三军医大学新桥医院胸外科,重庆,400037;第三军医大学新桥医院胸外科,重庆,400037;第三军医大学新桥医院胸外科,重庆,400037 |
| |
| 摘 要： | 目的了解线粒体DNA突变在肿瘤发生发展中的作用.方法应用PCR-限制性片段长度多态性分析技术对LA795、CT26和SCC891等3个肿瘤细胞系mtDNA编码区基因片段进行了分析.结果 mtDNA编码区的tRNAMet Glu Ⅰle及NDI基因核酸片段经HaeⅢ等16种内切酶消化后,这些肿瘤细胞系表现出完全相同的酶切图谱,其酶切格局及酶切片段大小完全相同.结论本组肿瘤细胞系mtDNA编码区基因结构相对稳定.
|
| 关 键 词： | 鼠源性肿瘤 线粒体DNA 限制性片段长度多态性 |
| 文章编号： | 1671-8348(2004)11-1674-03 |
The RFLP analysis on mutation of mtDNA from mouse tumors |
| |
| DAI Ji-gang,WEI Hong,MIN Jia-xin.The RFLP analysis on mutation of mtDNA from mouse tumors[J].Chongqing Medical Journal,2004,33(11):1674-1675,1678. |
| |
| Authors: | DAI Ji-gang WEI Hong MIN Jia-xin |
| |
| Abstract: | Objective To inquire into the relationship between mutations of mtDNA and carcinogenesis.Methods The variations of ND3 and tRNA Met Glu Ile gene fragments of mtDNA from three tumor cell lines of mice was analyzed by PCR-RFLP techniques.Results After being cleavaging by 16 restriction endonucleases,all restriction patterns of ND3 and tRNA Met Glu Ile gene fragments were same in these tumors.Conclusion The gene structure of coding region of mitochondrial DNA from these tumors is comparatively conserved. |
| |
| Keywords: | mitochondrial DNA tumor RFLP |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
