Onset of Coronary Heart Disease is Associated with HCMV Infection and Increased CD14~+CD16~+ Monocytes in a Population of Weifang,China

Objective To investigate the relationship between human cytomegalovirus(HCMV) infection and peripheral blood CD14~+CD16~+ monocytes in the pathogenesis of coronary heart disease(CHD), and to elucidate the mechanism of pathogenesis in CHD by analyzing the correlation between infection,inflammation, and CHD, to provide a basis for the prevention, evaluation, and treatment of the disease.Methods In total, 192 patients with CHD were divided into three groups: latent CHD, angina pectoris,and myocardial infarction. HCMV-IgM and-IgG antibodies were assessed using ELISA; CD14~+CD16~+monocytes were counted using a five-type automated hematology analyzer; mononuclear cells were assessed using fluorescence-activated cell sorting; and an automatic biochemical analyzer was used to measure the levels of triglyceride, cholesterol, high-and low-density lipoprotein cholesterols,lipoprotein, hs-CRp and Hcy.Results The positive rates of HCMV-IgM and-IgG were significantly higher in the CHD groups than in the control group. HCMV infection affects lipid metabolism to promote immune and inflammatory responses.Conclusion HCMV infection has a specific correlation with the occurrence and development of CHD.The expression of CD14~+CD16~+ mononuclear cells in the CHD group was increased accordingly and correlated with acute HCMV infection. Thus, HCMV antibody as well as peripheral blood CD14~+CD16~+mononuclear cells can be used to monitor the occurrence and development of CHD.