Genetic Diversity,Antimicrobial Resistance,and Virulence Genes of Aeromonas Isolates from Clinical Patients,Tap Water Systems,and Food |
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Institution: | 1. State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 10026, China;2. Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang 310058, Hangzhou, China;3. Ma’anshan Center for Disease Control and Prevention, Ma’anshan 243031, Anhui, China;4. Clinical Laboratory of Xuanwu Hospital, Captital Medcial University, Beijing 100053, China |
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Abstract: | ObjectiveThis study aimed to evaluate the genetic diversity, virulence, and antimicrobial resistance of Aeromonas isolates from clinical patients, tap water systems, and food.MethodsNinety Aeromonas isolates were obtained from Ma’anshan, Anhui province, China, and subjected to multi-locus sequence typing (MLST) with six housekeeping genes. Their taxonomy was investigated using concatenated gyrB-cpn60 sequences, while their resistance to 12 antibiotics was evaluated. Ten putative virulence factors and several resistance genes were identified by PCR and sequencing.ResultsThe 90 Aeromonas isolates were divided into 84 sequence types, 80 of which were novel, indicating high genetic diversity. The Aeromonas isolates were classified into eight different species. PCR assays identified virulence genes in the isolates, with the enterotoxin and hemolysin genes act, aerA, alt, and ast found in 47 (52.2%), 13 (14.4%), 22 (24.4%), and 12 (13.3%) of the isolates, respectively. The majority of the isolates (≥ 90%) were susceptible to aztreonam, imipenem, cefepime, chloramphenicol, gentamicin, tetracycline, and ciprofloxacin. However, several resistance genes were detected in the isolates, as well as a new mcr-3 variant.ConclusionsSequence type, virulence properties, and antibiotic resistance vary in Aeromonas isolates from clinical patients, tap water systems, and food. |
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Keywords: | Multi-locus sequence typing Multidrug resistance Virulence gene Antimicrobial resistance gene |
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