| 脑铁调素增高诱导膜铁转运蛋白1及其mRNA的表达下调 |
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| 引用本文： | 陈兵兵,李琳. 脑铁调素增高诱导膜铁转运蛋白1及其mRNA的表达下调[J]. 山西医科大学学报, 2007, 38(9): 793-796 |
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| 作者姓名： | 陈兵兵 李琳 |
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| 作者单位： | 山西医科大学老年医学研究所,太原,030001 |
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| 摘 要： | 目的研究脑室注射铁调素(hepcidin,Hep)对膜铁转运蛋白1(ferroportin1,FP1)及其mRNA表达的调节。方法雄性Wistar大鼠16只,随机分为FP1组及对照组,FP1组脑室注射Hep,对照组脑室注射无菌生理盐水,12h后,分别用免疫组化和RT-PCR法测皮质、海马的FP1及其mRNA的表达。结果脑皮质和海马FP1及其mRNA表达较对照组均明显减少(P<0.01)。结论Hep可直接抑制FP1及其mRNA的表达。
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| 关 键 词： | 铁调素 膜铁转运蛋白1 免疫组化 逆转录聚合酶链反应 |
| 文章编号： | 1007-6611（2007）09-0793-04 |
| 修稿时间： | 2007-05-08 |
Downregulation of ferroprtin 1 and its mRNA expression induced by hepcidin |
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| CHEN Bing-bing,LI Lin. Downregulation of ferroprtin 1 and its mRNA expression induced by hepcidin[J]. Journal of Shanxi Medical University, 2007, 38(9): 793-796 |
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| Authors: | CHEN Bing-bing LI Lin |
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| Affiliation: | Institute of Gerontology, Shanxi Medical University, Taiyuan 030001, China |
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| Abstract: | Objective To study the expression of FP1 and its mRNA induced by injecting hepcidin(Hep) into lateral cerebral ventricle. Methods Sixteen male Wistar rats were randomly divided into 2 groups: FP1 group(injected with Hep) and control group(injected with sodium chloride injection). The expression of FP1 and FP1 mRNA in the cortex and hippocampus of rats after injecting into lateral cerebral ventricle for 12 h were measured by immunohistochemistry and RT-PCR. Results The expression of FP1 and its mRNA all decreased significantly in cerebral cortex and hippocampus after injecting Hep(P<0.01) . Conclusion Hep could directly inhibit the expression of FP1 and FP1 mRNA. |
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