输血后肝炎患者、血透患者及非甲～戊型肝炎患者中庚型肝炎病毒感染的调查

目的：确定广州地区输血后肝炎患者、血透患者、急性非甲～戊型肝炎患者及对照组中庚型肝炎病毒（ＨＧＶ）的感染状况。方法：根据ＨＧＶ感染者５’－非编码区的核苷酸序列设计引物，应用逆转录套式聚合酶链反应（ＲＴ－ｎｅｓｔｅｄ－ＰＣＲ）对庚型肝炎病毒核糖核酸（ＨＧＶ－ＲＮＡ）进行检测。结果：共检测３６０价标本，上述各组中ＨＧＶ感染率分别为２２．６％、１４．８％、４．０％及２．５％。结论：输血后肝炎患者、血透患者及急性非甲～戊肝炎患者中ＨＧＶ感染率显著高于对照（Ｐ值均＜０．００５）；有血液接触史者高于无血液接触史者（ Ｐ值＜０、 ００５）；ＨＧＶ－ＲＮＡ与抗－ＨＧＶ无平行关系。

An Investigation of Hepatitis G Virus Infection in Patients with Post-transfusion Hepatitis,Hemodialysis and Non A-E Hepatitis

Objectives:To investigate the situation Of hepatitis G virus (HGV) infection among patients with post-trans fusion hepatitis, hemedialysis, non A-E hepatitis and normal control in Guangzhou area. Methods: A set of reverse- nested- polymerase chain react ion (RT- nested -PCR ) was conducted by using primers designed according to the 5'-noncoding sequence in patients with HGV infection, to detect the RNA of HGV (HGV - RNA). Results: Totally 360 samples were assayed, the infec- tion rates of HGV in the above groups were 22. 6 %, 14. 8 %, and 2. 5 %, respectively. Conclusion: The infection rates of HGV in patients of post-transfusion hepatitis, hemedialysis and acute non A -E hepatitis were higher than that in the control (P < 0. 005 ); That in patients with the history of blood exposure was obviously higher than that in patients without the history(P< 0. 005 ); There appeared to be no parallel relationship between levels of HGV- RNA and anti - HGV.