The vimentin-tubulin binding site peptide (Vim-TBS.58-81) crosses the plasma membrane and enters the nuclei of human glioma cells |
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Authors: | Balzeau Julien Peterson Alan Eyer Joel |
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Institution: | a Laboratoire Neurobiologie & Transgenese, UPRES-EA3143, Université d’Angers, Bâtiment IBS-IRIS, Centre Hospitalier Universitaire, 49033 Angers, France b Laboratory of Developmental Biology, McGill University, Royal Victoria Hospital, H5-35, 687 Pine Av. W, H3A1A1 Montreal, Canada |
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Abstract: | Cell-penetrating peptides (CPPs) can translocate through the plasma membrane and localize in different cell compartments providing a promising delivery system for peptides, proteins, nucleic acids, and other products. Here we describe features of a novel cell-penetrating peptide derived from the intermediate filament protein vimentin, called Vim-TBS.58-81. We show that it enters cells from a glioblastoma line via endocytosis where it distributes throughout the cytoplasm and nucleus. Moreover, when coupled to the pro-apoptogenic peptide P10, it localizes to the nucleus inhibiting cell proliferation. Thus, the Vim-TBS.58-81 peptide represents an effective vector for delivery of peptides and potentially a broad range of cargos to the nucleus. |
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