| 川芎嗪对MPTP诱导的多巴胺能神经元毒性的影响 |
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| 引用本文： | 杜静,单璐琛,张高小,王玉强. 川芎嗪对MPTP诱导的多巴胺能神经元毒性的影响[J]. 时珍国医国药, 2011, 22(7). DOI: 10.3969/j.issn.1008-0805.2011.07.007 |
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| 作者姓名： | 杜静 单璐琛 张高小 王玉强 |
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| 作者单位： | 暨南大学药学院新药研究所,广东,广州,510632 |
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| 基金项目： | 国家自然科学基金，国家重大新药创制 |
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| 摘 要： | 目的 观察川芎嗪体外、体内对MPTP)导致的多巴胺能(dopaminergic,DA)神经元毒性的影响.方法 体外实验,提取孕14 d的Sprague-dawley大鼠胎鼠黑质区多巴胺神经元,MPP<'+>诱导其损伤,运用免疫荧光法测定黑质酪氨酸羟化酶(tyrosine hydroxylase,TH)阳性神经元数量及状态变化;体内实验,以C57BL/6J小鼠为研究对象,采用腹腔注射MPTP方法制作小鼠慢性帕金森(Parkinson's disease,PD)模型,运用免疫组化法测定TH阳性神经元数量变化.结果 体外实验:TMP组多巴胺神经元形态数目均有所改善,其中500μmol/L组与模型组相比具显著性差异.体内实验:与模型组相比,注射TMP后,多巴胺神经元密度有所增加.结论 TMP能不同程度地改善MPTP诱导的多巴胺神经元的损伤.
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| 关 键 词： | 川芎嗪 多巴胺神经元 |
Effect of TMP on Dopaminergic Neuron Injury Induced by MPTP in vivo and vitro |
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| DU Jing,SHAN Lu-chen,ZHANG Gao-xiao,WANG Yu-qiang. Effect of TMP on Dopaminergic Neuron Injury Induced by MPTP in vivo and vitro[J]. Lishizhen Medicine and Materia Medica Research, 2011, 22(7). DOI: 10.3969/j.issn.1008-0805.2011.07.007 |
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| Authors: | DU Jing SHAN Lu-chen ZHANG Gao-xiao WANG Yu-qiang |
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| Affiliation: | DU Jing,SHAN Lu-chen,ZHANG Gao-xiao,WANG Yu-qiang(Institute of New Drug Research,College of Pharmacology,Jinan University,Guangzhou,510632,China) |
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| Abstract: | ObjectiveTo investigate the effect of tetramethylpyrazine(TMP) on dopaminergic neuron injury induced by N-methyl-4-phenyl-1,2,3,6-tetrahydro-pyridine(MPTP) in vitro and in vivo.MethodsIn vitro: Rat mesencephalic neurons were cultured for 5 days,followed by addition of MPP+.The number and morphology of positive TH neurons were observed using an inverted fluorescence microscope.In vivo: Mice were administered MPTP(25 mg/kg,i.p,per day) for 5 days to establish the PD animal model.The number of positive TH cell... |
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| Keywords: | MPP<'+> |
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