| HPLC测定蓝菊抗病毒口服液中蒙花苷的含量 |
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| 引用本文： | 郑艳平,林壮民.HPLC测定蓝菊抗病毒口服液中蒙花苷的含量[J].中国民族民间医药杂志,2011,20(16):45-46. |
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| 作者姓名： | 郑艳平 林壮民 |
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| 作者单位： | 广东省第二人民医院,广东,广州,510317 |
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| 基金项目： | 蓝菊抗病毒口服液工艺及质量标准研究 |
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| 摘 要： | 目的：建立蓝菊抗病毒口服液中蒙花苷的含量测定方法。方法：采用HPLC法，色谱柱：Acclaiml20C18柱（4．6mm×250mm，5μm）；流动相：甲醇-水-冰醋酸（26：23：1）；柱温：25℃；检测波长为334nm；流速为1．0mL·min^-1。结果：蒙花苷在O．025—0．25μg之间线性关系良好，R^2=0．9999，平均加样回收率为102．41％（RSD=1．5％，n=6）。结论：本方法简便、准确，重复性好，可用于该制剂的质量控制。
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| 关 键 词： | 蒙花苷 蓝菊抗病毒口服液 HPLC |
Determination of linarin in Lanju Kangbingdu oral liquid by HPLC |
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| Zheng Yan-ping,Ling Zhuang-min.Determination of linarin in Lanju Kangbingdu oral liquid by HPLC[J].Chinese Journal of Ethnomedicine and Ethnopharmacy,2011,20(16):45-46. |
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| Authors: | Zheng Yan-ping Ling Zhuang-min |
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| Institution: | Zheng Yan-ping,Ling Zhuang-minThe second People's Hospital of Guangdong Province,Guangzhou 510317,China |
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| Abstract: | Objective： To establish a method for the content determination of linarin in Lanju Kangbingdu oralliquid by HPLC. Methods： The content of adenosine was determined by HPLC and the chromatographic conditions were： Acclaim 120 C18 column （4. 6mm ×250mm, 5μm） ; the mobile phase consisting of methanol - water - glacial acetic acid slution （26： 23：1 ） ; column tem- perature at 25℃ ; detection wavelength being 334nm; flow rate 1.0 mL · min^-1 ; Results： A good linearity of linarin was in the range of O. 025 μg - 0. 25μg （ R^2 =0. 9999） ; The average recovery rate was 102. 41%, RSD = 1.5 %. Conclusion： The method is simple and accurate and with a good reproducibility and can be used for the quality control of Lanju Kangbingdu oraUiquid. |
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| Keywords: | HPLC |
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