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| 贵州忍冬花叶茎中绿原酸含量的比较 | |
| 引用本文： | 王颖,王华林,张文,罗天允,周英.贵州忍冬花叶茎中绿原酸含量的比较[J].时珍国医国药,2011,22(7):1606-1607. |
| 作者姓名： | 王颖 王华林 张文 罗天允 周英 |
| 作者单位： | 1. 贵州大学生命科学学院,贵州,贵阳,550025 2. 贵州大学理学院,贵州,贵阳,550025 3. 贵州大学生命科学学院,贵州,贵阳,550025;贵州省中药材繁育与种植工程实验室,贵州,贵阳,550025 |
| 基金项目： | 贵州省国际科技合作重点项目 |
| 摘 要： | 目的对贵州忍冬叶中绿原酸的提取工艺进行了优化,同时比较不同生长时期忍冬花、叶、茎中绿原酸的含量。方法采用超声波法提取绿原酸,并通过正交法确定其最佳提取参数。结果最佳提取参数为:70%乙醇,料液比为1∶15,样品预浸24 h和提取时间10 min。在此条件下对忍冬花、叶、茎中的绿原酸分别进行提取测定,得到忍冬叶中绿原酸的含量高于花中的含量,且越冬老叶中为最高,茎中含量极少。结论该正交实验法所得条件,能对绿原酸进行高效地提取,稳定性好;初步认为贵州地区忍冬叶具有较高的开发利用价值。 |
| 关 键 词： | 忍冬 绿原酸 提取优化 |
Comparison of Chlorogenic Acid Content in Flowers,Leaves and Stems of Guizhou Lonicera Japonica Thunb |
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| WANG Ying,WANG Hua-lin,ZHANG Wen,LUO Tian-yun,ZHOU Ying.Comparison of Chlorogenic Acid Content in Flowers,Leaves and Stems of Guizhou Lonicera Japonica Thunb[J].Lishizhen Medicine and Materia Medica Research,2011,22(7):1606-1607. | |
| Authors: | WANG Ying WANG Hua-lin ZHANG Wen LUO Tian-yun ZHOU Ying |
| Institution: | WANG Ying1,WANG Hua-lin2,ZHANG Wen2,LUO Tian-yun2,ZHOU Ying1,3(1.College of Life Science,Guizhou University,Guiyang,550025,China,2.College of Science,55025,3.Guizhou Engineering Laboratory of Propogdia and Cultivation on Traditional Chinese Medicina Materials,China) |
| Abstract: | ObjectiveTo obtain the optimum extraction method for chlorogenic acid from the leaves of Guizhou Lonicera japonica Thunb.,and compare the chlorogenic acid content in the flower,leaves,stems during different growth periods.MethodsUltrasound method was used to extract chlorogenic acid in Lonicera,and orthogonal method was adopted to determine the optimum parameters for the extraction.ResultsThe optimum parameters for the extraction were as follows: 70% ethanol,15 times,samples prepreg 24h and extraction time ... |
| Keywords: | Lonicera japonica Thunb Chlorogenic acid Extraction |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |