| 高表达整合素连接激酶对大鼠骨髓间充质干细胞旁分泌功能的影响 |
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| 引用本文： | 高见书,林承曦,毛庆,康丽娜,徐标.高表达整合素连接激酶对大鼠骨髓间充质干细胞旁分泌功能的影响[J].中国动脉硬化杂志,2011,19(5):380-384. |
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| 作者姓名： | 高见书 林承曦 毛庆 康丽娜 徐标 |
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| 作者单位： | 南京大学医学院附属鼓楼医院心内科,江苏省南京市,210008 |
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| 基金项目： | 国家自然科学基金，江苏省自然科学基金 |
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| 摘 要： | 目的 研究转染腺病毒介导的整合素连接激酶(ILK)对大鼠骨髓间充质干细胞旁分泌作用的影响.方法 构建携带人野生型整合素连接激酶质粒cDNA和人重组绿色荧光蛋白的腺病毒载体(adeno-ILK)和空载腺病毒(MSC),通过全骨髓贴壁法分离培养骨髓间充质干细胞.采用无血清培养转染adeno-ILK(MSC-ILK组)或空载腺病毒(MSC组),通过流式细胞术检测人重组绿色荧光蛋白从而测定转染效率,确定最佳的病毒感染复数.提取骨髓间充质干细胞mRNA,用Real-time PCR测定血管内皮生长因子、成纤维细胞生长因子2和胰岛素样生长因子1的基因表达量.结果 MSC-ILK组旁分泌因子血管内皮生长因子、成纤维细胞生长因子2和胰岛素样生长因子1的基因表达量分别是MSC组的 8.2±0.4、2.6±0.2及2.4±0.2倍(P＜0.05或P＜0.01).结论 整合素连接激酶基因转染可明显促进骨髓间充质干细胞表达旁分泌因子血管内皮生长因子、成纤维细胞生长因子2和胰岛素样生长因子1.
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| 关 键 词： | 整合素连接激酶 骨髓间充质干细胞 血管内皮生长因子 成纤维细胞生长因子2 胰岛素样生长因子1 |
| 收稿时间： | 2011/3/27 0:00:00 |
Effects of Transfection of Integrin-Linked Kinase Gene to Bone Mesenchymal Stem Cells on the Paracrine Action in Rats |
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| GAO Jian-Shu,LIN Chen-Xi,MAO Qing,KANG Li-N,and XU Biao.Effects of Transfection of Integrin-Linked Kinase Gene to Bone Mesenchymal Stem Cells on the Paracrine Action in Rats[J].Chinese Journal of Arteriosclerosis,2011,19(5):380-384. |
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| Authors: | GAO Jian-Shu LIN Chen-Xi MAO Qing KANG Li-N and XU Biao |
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| Institution: | GAO Jian-Shu,LIN Chen-Xi,MAO Qing,KANG Li-Na,and XU Biao (Department of Cardiology,Drum Tower Hospital,Nanjing University Medical School,Nanjing,Jiangsu 210008,China) |
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| Abstract: | Aim To study the effect of transfection of adenovirus-mediated integrin-linked kinase(ILK) on rat bone marrow mesenchymal stem cell(BMSC) paracrine action.Methods BMSC were isolated and cultured in vitro by adherent culture.The recombinant adenoviral vector was constructed containing both human wild-type ILK cDNA and humanized recombinant green fluorescent protein(hrGFP).Corresponding virus with null content(adeno-null) was used as a control.BMSC were infected by adeno-ILK(MSC-ILK group) or adeno-null(MSC g... |
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| Keywords: | Integrin-Linked Kinase Bone Marrow Mesenchymal Stem Cells Vascular Endothelial Growth Factor Fibroblast Growth Factor-2 Insulin-Like Growth Factor-1 |
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