Shear activated channels in cell-attached patches of cultured bovine aortic endothelial cells |
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| Authors: | Elizabeth R. Jacobs Christine Cheliakine Debebe Gebremedhin Eric K. Birks Peter F. Davies David R. Harder |
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| Affiliation: | (1) Departments of Physiology and Medicine MCW Clinic at Froedtert, Medical College of Wisconsin, 9200 West Wisconsin Avenue, 53226 Milwaukee, WI, USA;(2) Faculté des Sciences et Techniques Laboratoires D'electrophysiologie Cellulaires, Université de Tours, F-37200 Paro de Grandmont, Tours, France;(3) Department of Pathology, University of Chicago, M.C. 6079, 5841 South Maryland Avenue, 60637-1470 Chicago, IL, USA |
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| Abstract: | We investigated the response of inward rectifier K+ (IRK) currents in bovine aortic endothelial cells (BAECs) to shear stress. Shear evoked reversible hyperpolarization in current clamped BAECs. Voltage clamped BAECs exhibited large inward and small outward whole cell K+ currents blocked by cesium and increased in amplitude by exposure to shear stress. The open state probability of IRK channels in cell-attached membrane patches was increased within minutes of exposure to shear stress. IRK channels in inside-out patches were activated by increases in [Ca2+]i from 10–7 to 10–6 mM. We demonstrate that shear stress induces hyperpolarization and gating of single channel and whole cell IRK currents in BAECs.Supported by NIH grant numbers K08-1924, HL49294 (ERJ), HL 29587-09 (DH), R37 HL 33833-07 (DH), and HL15062 (PFD). |
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| Keywords: | Potassium channels membrane potential hyperpolarization endothelial cells shear stress inward rectifier ion channels calcium sensitivity |
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