神经营养因子NGF、BDNF对AD模型鼠海马移植的影响

目的:研究神经营养因子NGF、BDNF对AD模型鼠海马移植后行为和形态学变化。方法:24.只AD模型鼠随机分成4组:单纯胚基底前脑细胞悬液移植组(ST组)、含NGF或BDNF胚基底前脑细胞悬液移植组(NGF组)、(BD-NF组)和模型对照组(M组),移植后3月进行行为测试并比较移植区AchE细胞数和纤维密度,运用方差分析和SNK检验进行组间比较。结果:行为测试移植3组明显优于模型M组,含因子组又较ST组效果好(P<0.01),两因子组间差异无显著性(P>0.05);因子组存活细胞数均高于ST组,NGF组细胞数多于BDNF组(P<0.05),纤维密度两组相似(P>0.05)。结论:海马内存活胆碱能神经元能代偿受损胆碱能神经元的功能,改善动物的学习记忆功能;NGF、BDNF均能促进胆碱能神经元存活,增加AchE细胞数目和突起,但BDNF促进神经元突起延伸作用较好,而NGF则对神经元保护作用较强。

Effects of NGF and BDNF on Hippocampal Transplantation in AD Rats

Objective; to investigate the changes of behaviour and morphology following hippocampal transplantation in AD rats and the effects of NGF ,BDNF on them Methods; twenty-four AD models were randomly divided into four groups ;the group Ⅰ (ST group) , simply implanted fetal basal forebrain suspension into the lesioned hippocampus, group Ⅱ ( NGFgroup) and group Ⅲ ( BDNFgroup) , cell suspension containing NGF or BDNF respectively, and group IV lesioned rats served as a model control group (M group) . three months after transplantation ,the behaviour tests were carried out and the number of AchE-positive neurons and the density of AchE-positive fibre in graft areas were compared, the statistical data were treated by ANOVA and SNK test Results: the behaviour tests showed that ability of learning and memory in three grafted groups were significant difference with the M group (P <0. 01) ,and the group containing neurotrophic factors NGF and BDNF were better than ST group (P <0. 01) ,no significant difference was found between NGF group and BDNF group(P >0. 05) , the number of AchE-positive neurons and the density of AchE-positive fibre in graft areas of neurotrophic factors groups were more than that of ST group( p < 0. 01) , in addition there were more neuronsin NGF group and longer processes in BDNF group. Conclusions; the implanted cholinergic neurons could ameliorate the behaviour impairment of tearing and memory . either NGF or BDNF could promote the survival of transplanted cholinergic neurons ,but BDNF had better effect on neurites extending, while NGF had the better ability in protecting the cholingic neurons.