氧化应激在大鼠肢体缺血再灌注后心肌损伤中的作用

①目的 观察大鼠肢体缺血再灌注(LIR)后心肌的损伤变化。②方法 将实验大鼠随机分为7组，即对照组(control)、缺血组(1)、缺血再灌注0．5小时组(R0.5)、2小时组(R2)、4小时组(R4)、6小时组(R6)、10小时组(R10)。分别测定血象及心肌组织中丙二醛(MDA)浓度和超氧化物歧化酶(SOD)水平，并测定心肌组织髓过氧化物酶(MPO)和血浆乳酸脱氢酶(LDH)活性。③结果 与对照组比较，MDA、LDH、MPO检测量在R0.5、R2、R4组依次增加，在R6、R10两组依次减少；SOD活性在R0.5、R2、R4组依次下降，在R6组有所恢复，至R10组又有所下降。④结论 大鼠肢体缺血再灌注(IR)后继发心肌损伤，可能与氧化应激有关。

The injury changes of myocardial after limb ischemia -reperfusion in rats

Objective To observe the changes of myocardial injury after limb ischemia reperfusion (IR) in rats in different reperfusion periods. Methods Wistar rats were randomly divided into 7 groups :control group, I group, R 0.5 group, R 2 group, R 4 group ,R 6 group and R 10 group.On the model of limb ischemia reperfusion in rats ,the concentrations of malondialdehyde(MDA) and superoxide dismutase (SOD) in the plasma and myocardial tissue ,the level of lactate dehydrogenase (LDH) in the plasma and myeloperoxidase(MPO) in the myocardial tissue were meassured. Results It was found that compared with, the control group, the values of MDA , LDH and MPO shows a tendency that they increased in R 0.5 group, R 2 group and R 4 group in order and decreased in R 6 group and R 10 group . However, the level of SOD in R 0.5 group,R 2group and R 4group decreased gradually but increased in R 6group temporarily and it decreased in R 10group again. Conclusion The myocardial injury occurred after limb ischemia-reperfusion which may be related to oxidation.