近似人病理生理过程动物脂肪肝模型的综合建立方法

目的建立近似人病理生理过程的动物脂肪肝模型，使之适用于影像学定量诊断或药物疗效的验证研究。方法健康新西兰大白兔45只，随机分为四组：正常对照组10只，普通饲料及水喂养9周；实验1、2、3组，各10只，采用高脂高糖饲料＋酒精饮料分别喂养3周、6周和12周；补充组5只，给予实验组相同的喂养和处理。采用HE染色和苏丹Ⅳ染色对动物肝脏进行组织学检查。结果实验成模率为87％（39／45），死亡率为13％（6／45），出现正常肝10只，轻度脂肪肝10只，中度脂肪肝9只，重度脂肪肝10只（HE染色），与苏丹Ⅳ染色Vv值分级的诊断符合率约为75％。结论综合运用高脂高糖饲料＋酒精饮料可成功建立近似人病理生理过程的动物脂肪肝模型。

Synthetical establishment method of fatty liver model similar with human pathophysiology

Objective To establish animal model of fatty liver similar with human pathophysiology used for the study of imaging quantitative diagnosis or verification of medicine curative effect. Methods Forty-five New Zealand white rabbits were used in this study. They were divided randomly into five groups : 10 rabbits in normal control group, 10 rabbits in experiment group 1,10 rabbits in experiment group 2,10 rabbits in experi-ment group 3,and remaining 5 rabbits were in a supplement group. Rabbits in normal control group were given free access to normal food and water for 9 weeks. Rabbits in the experimental groups were fed a high-fat and high-sucrose diet for 3,6,and 12 weeks,respectively. Rabbits in the supplement group were fed the same diet as the experimental groups. Liver specimen stained with HE and Sudan Ⅳ was examined histological. Results The success rate of hepatic steatotie models was 87 % (39 of 45 ) ;mortality was 13 % (6 of 45 ). There were 10 nor-mal livers, 10 mildly fatty livers, 9 moderately fatty livers, and 10 severely fatty livers in results (stained with HE), and the rate of conforming to Sudan Ⅳ was 75%. Conclusion The animal model of fatty liver with differ-ent severity can be established by being fed a high fat, high sucrose and alcohol diet.