hTERT CD 44v6 在大肠癌中的表达及意义

目的：探讨hTERT 、CD44v6 的表达与大肠癌发生、浸润转移及预后的关系。方法：对大肠腺瘤组织及大肠癌组织进行免疫组织化学染色，分析hTERT 及CD44v6 在大肠癌中表达的相关性。结果：hTERT 及CD44v6 在大肠癌组织中的阳性表达率分别为70.73% 、53.66% ，明显高于大肠腺瘤组织中的10.53% 、10.53%（P<0.01）；hTERT 及CD44v6 在有淋巴结转移的大肠癌患者的阳性表达率分别为93.33% 、86.67% ，明显高于无淋巴转移患者的阳性表达率53.85% 、38.46%（P<0.01）；hTERT 及CD44v6 在TNMⅢ、Ⅳ期大肠癌患者的阳性表达率分别为94.12% 、82.35% ，明显高于TNMⅠ、Ⅱ期的54.17% 、33.33%（P<0.01）；在生存期<5 年大肠癌中的阳性表达率分别为90.91% 、77.27% ，明显高于生存期≥5 年的47.37% 、26.32%（P<0.01）；hTERT 和CD44v6 在性别、年龄、肿物大小、肿物位置及浸润深度中的阳性表达差异均无统计学意义（P>0.05）。 结论：hTERT 和CD44v6 在大肠癌组织中具有高表达，特别是在有淋巴结转移、TNMⅢ、Ⅳ期和生存期<5 年组中有较高表达，说明两者可能在大肠癌的发生、浸润转移过程中起着重要作用，并且与大肠癌患者的预后有关；联合检测hTERT 和CD44v6 可以作为大肠癌浸润转移的判断和预后评估的参考指标。

The Expression and Significance of hTERT and CD44v6 in Colorectal Carcinoma

Objective: To explore the relationship of hTERT and CD 44v6 expression with the carcinogenesis, infiltra-tion, metastasis, and prognosis of colorectal carcinoma. Methods:Immunohistochemistry was used to detect the expres -sion of hTERT and CD44v6 in the tissue of colorectal carcinoma and adenoma. Results: The positive rates of expression of hTERT and CD 44v6 in colorectal carcinoma tissue were 70.73% and 10.53%, respectively; the positive rates of expression of hTERT and CD 44v6 were10.53% and 10.53% in colorectal adenoma tissue, respectively, with a significant difference be-tween the 2 groups ( P<0.01). The positive rates of expression of hTERT and CD 44v6 in colorectal carcinoma tissue from patients with lymph node metastasis were respectively 93.33% and 86.67%, distinctly higher than those from patients with-out lymph node metastasis ( 53.85% and 38.46%), with a significant difference (P<0.01). The positive rates of hTERT and CD44v6 expression in III and IV stage colorectal carcinoma tissue were 94.12% and 82.35%, higher than those in TNM I and II stage colorectal carcinoma tissue (54.17% and 33.33%), with a significant difference (P< 0.01). The positive rates of hTERT and CD44v6 expression in colorectal carcinoma patients with survival of less than 5 years were 90.91% and 77.27% , distinctly higher than those in patients with survival of5 or more years (P<0.01). Expression of hTERT and CD44v6 was not correlated with sex, age, tumor size, tumor location, or depth of infiltration (P>0.05). Conclusion:High ex-pressions of hTERT and CD 44v6 exist in colorectal carcinoma, especially in the tissue from patients with lymph node me-tastasis, TNM stages III and IV, and survival of fewer than 5 years. hTERT and CD 44v6 probably play an important role in the carcinogenesis, invasion, and metastasis of colorectal carcinoma. hTERT and CD 44v6 could be an index for the evalua-tion of infiltration, metastasis, and prognosis of colorectal carcinoma.