蛴螬重金属及有害元素的分析

目的: 分析不同产地蛴螬中重金属及有害元素的含量,评价蛴螬的药用安全性,为药材标准的制定提供试验依 据。 方法: 采用微波消解/电感耦合等离子体质谱（ICP-MS）法和原子吸收法同时测定了5个不同产地蛴螬中铅、镉、砷、汞、铜的含量;并以《中国药典》2010年版一部限量标准为依据,判断蛴螬中重金属及有害元素含量超标情况。 结果: 两种方法测定的铅、镉、砷、汞、铜含量无统计学差异。ICP-MS法测定的5批蛴螬中铅平均含量为3.912 mg·kg^-1,镉为0.163 mg·kg^-1,砷为1.983 mg·kg^-1,汞为0.260 mg·kg^-1,铜为17.386 mg·kg^-1。原子吸收法测定的铅含量为3.777 mg·kg^-1,镉为0.155 mg·kg^-1, 砷为2.044 mg·kg^-1,汞为0.252 mg·kg^-1,铜为18.027 mg·kg^-1。 结论: 5批不同产地的蛴螬样品中镉含量均低于《中国药典》2010年版一部规定的限量标准;部分产地的蛴螬样品中铅、铜、砷、汞的含量超过《中国药典》的限量标准。研究从药用安全性角度为蛴螬药材标准的制订提供了参考。

Analysis of Heavy Metals and Harmful Elements in Holotrichia diomphalia

Objective: To analyze the content of heavy metals and harmful elements in Holotrichia diomphalia and provide the scientific foundation for the quality control and standard establishment of H. diomphalia. Method: The content of Pb,Cd,Cu,As,Hg constituents was determined by ICP/MS after microwave-assisted digestion and atom absorption spectrophotometry. The Chinese pharmacopoeia(Volume 1) of 2010 edition was used as the standard to evaluate the pollution condition of Pb,Cd,Cu,As,Hg in H. diomphalia. Result: The content of Pb,Cd,Cu,As,Hg constituents determined by ICP/MS had not obvious difference with the ones determined by atomabsorption spectrophotometry. The content of Pb,Cd,Cu,As and Hg determined by ICP/MS was 3.912,0.163,17.386,1.983 and 0.260 mg· kg^-1 respectively. The content of Pb,Cd,Cu,As and Hg determined by atom absorption spectrophotometry was 3.777,0.155,18.027,2.044 and 0.252 mg· kg^-1 respectively. Conclusion: The concentrations of Cd in the samples from five different areas of H. diomphalia were lower than the limits of Chinese pharmacopoeia,but the concentrations of Pb,Cu,As,Hg in the some samples from different areas were more than the limit standard. The result is helpful to develop the quality standards of H. diomphalia four harmful elements.