过氯酸铵对肺组织损害作用的研究

目的　研究过氯酸铵 (AP)对肺组织的损害作用 ,探讨AP有无致肺纤维化作用。方法　Wistar大鼠经气管内注入AP染毒一定时间后 ,取大鼠肺泡灌洗液 (BALF)和肺组织 ,测定细胞总数、肿瘤坏死因子 α(TNF α)、丙二醛 (MDA)、羟脯氨酸 (HYP)和胶原纤维等指标。结果　AP进入肺组织后能引起急性肺损伤、肺炎症反应 ;染毒后不同时间AP各组BALF中TNF α值均显著高于阴性对照组 (P <0 0 5 )。AP对MDA、HYP和胶原蛋白合成有一定影响 ;没有明显的肺纤维化病理变化。结论　AP对肺组织有急性损伤作用。本实验尚不能证明AP有致肺纤维化作用

Study on the injury effect of ammonium perchlorate to lung

OBJECTIVE: To study the injury effect of ammonium perchlorate (AP) to lung and to explore whether AP can cause pulmonary fibrosis. METHODS: To detect the levels of cell counts, TNF-alpha, MDA, HYP and the synthesis of collagen in BALF or rat lung after a certain time when rats were injected AP by intratracheal instillation. RESULTS: AP could bring about acute lung damage and inflammatory reaction. The levels of TNF-alpha of different groups in different time were obviously higher than the normal control group(P < 0.05). AP could affect the levels of MDA, HYP and the synthesis of collagen. But it had no obviously pathological change of pulmonary fibrosis. CONCLUSION: There were acute injury effect about AP to lung, but this experiment could not make sure whether AP could cause pulmonary fibrosis.