大肠癌中Cyclin E表达及其意义

目的：研究大肠癌中Cyclin E表达及其意义。方法：采用流式细胞术（FCM)对30例大肠癌实体瘤的癌组织和远切端正常组织Cyclin E表达率及细胞增殖指标（PI，SPF）进行检测，将Cyclin E阳性表达率与大肠癌临床病理指标进行比较，PI，SPF进行相关性分析。结果：大肠癌肿瘤组织的Cyclin E的阳性表达率及PI，SPF明显高于远癌切端正常组织，与肿瘤的恶性程度，淋巴结转移及发生部位无显著相关，与肿瘤细胞的增殖活性相关，在增生活跃的肿瘤细胞中Cyclin E伯表达率高。结论；Cyclin E过表达参与了大肠癌的发生，Cyclin E表达率与传统的临床病理指标无关，但与细胞增殖活性有明显相关性。Cyclin E表达率可能成为另一个判断大肠癌预后的指标。

Expression of Cyclie E in colorectal carcinoma and its significance

Objective: To investigate the expression of Cyclin E in colorectal carcinoma and its significance.Methods: The Cyclin E expression and the cell proliferation(PI,SPF)in 30 cases of colorectal carcinomas (including tumors and normal tissues distant to the tumors)were respectively assayed with Flow Cytometry (FCM), and the relationships between the Cyclin E expressive rate and clinical pathologic features were compared. Correlaction between the Cyclin E expressive rate and the cell proliferation(PI, SPF) was analyzed too.Results: The Cyclin E positive rate and the cell proliferation in colorectal carcinoma were significantly higher than those of the distal normal tissues. As for the Cyclin E positive rate and the cell proliferation,no significant differences were found among the subgroups divided according to malignance, lymphatic metastasis and the site of tumor. There was a significant correlation between the Cyclin E positive rate and the cell proliferation.Conclusion: The Cyclin E overexpression plays an important role in the onset of colorectal carcinoma.The Cyclin E expressive rate is not consistent with the general clinical pathologic features,but with the cell proliferation. The Cyclin E expressive rate may be one of the potential prognostic indicators in coloretal carcinoma.