缺氧缺血性脑损伤新生大鼠脑组织水通道蛋白9实验研究表达

目的研究缺氧缺血性脑损伤（HIBD）后水通道蛋白9（AQP9）在脑组织中的表达变化规律,初步探讨AQP9与脑水肿的关系。方法采用HIBD动物模型,实施定量PCR（基因水平）和Wester-Blot（免疫印迹）方法,检测AQP9表达的变化和AQP9 mRNA的变化。结果 HIBD组AQP9的表达随缺血时间延长而表达增加,对照组AQP9表达无明显变化,且AQP9表达在脑缺血2~3 d表达最强。结论 HIBD时,随着缺氧缺血时间的延长,AQP9表达增强,至72 h达高峰。

AQP9 expression in brain tissue of rats with hypoxic-ischemic brain damage in rats

Objective To study the change pattern of AQP9 expression in the brain tissues after hy- poxie-ischemic brain damage and to discuss its relationship with brain edema. Methods HIBD animal model was established,and quantitative PCR and Western-Blot were used to determine the changes of AQP9 ex- pression and AQP9 mRNA. Results AQP9 expression had no obvious change in the control group, but in the HIBD group, AQP9 expression increased with the lasting time of ischemia, and it reached its highest level on the second and third day of ischemia. Conclusions In hypoxie-ischemic brain damage,AQP9 expression increases with the lasting time of hypoxia and ischemia,and it reaches its highest level at 72 hours.