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| 蜈蚣多糖对Hela细胞的增殖抑制作用研究 | |
| 引用本文： | 李兴暖,韩雅莉,余卫国.蜈蚣多糖对Hela细胞的增殖抑制作用研究[J].时珍国医国药,2009,20(7). |
| 作者姓名： | 李兴暖 韩雅莉 余卫国 |
| 作者单位： | 1. 九江学院基础医学院,江西,九江,332000 2. 广东工业大学,广东,广州,510090 3. 中国人民解放军91960部队医院,广东,汕头,515074 |
| 基金项目： | 国家自然科学基金，广东工业大学基金 |
| 摘 要： | 目的 研究蜈蚣多糖对人宫颈癌细胞Hela细胞的抑制作用及作用机制.方法 用MTT法检测蜈蚣多糖对Hela细胞生长的抑制作用;流式细胞仪和Annexin V-FITC/PI双荧光染色分别检测蜈蚣多糖对Hela细胞周期及凋亡情况的影响.结果 在一定浓度范围内蜈蚣多糖可显著抑制Hela细胞的增殖,改变Hela细胞的细胞周期,使细胞阻滞于G2/M期,并能诱导细胞凋亡.结论 蜈蚣多糖可能通过改变Hela细胞的细胞周期,诱导细胞凋亡,从而对Hela细胞的增殖有一定的抑制作用. |
| 关 键 词： | 蜈蚣多糖 细胞周期 细胞凋亡 |
Effects of Polysaccharide from Scolopendra subspinipes mutilans on Proliferation of Hela Cells |
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| LI Xing-nuan,HAN Ya-li,YU Wei-guo.Effects of Polysaccharide from Scolopendra subspinipes mutilans on Proliferation of Hela Cells[J].Lishizhen Medicine and Materia Medica Research,2009,20(7). | |
| Authors: | LI Xing-nuan HAN Ya-li YU Wei-guo |
| Abstract: | Objective To investigate the inhibitory effects of polysaccharide from Scolopendra subspinipes mutilans (PSSM) on Hela cells and its mechanism.Methods MTT assay was used to test the effect of growth inhibition on Hela cells.Cell cycle and cell apopotsis were detected by flow cytometry and AnnexinV-FITC/PI fluorescence staining.Results PSSM could obviously inhibit the proliferation of Hela cells at specific concentration range,change the cell cycle of Hela cells and block Hela cells at G2/M phase,and induce cell apoptosis.Conclusion PSSM can inhibit the growth of Hela cells by changing cell cycle and inducing apoptosis. |
| Keywords: | Polysaccharide from Scolopendra subspinipes mutilans Cell cycle Cell apoptosis |
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