来那度胺对人宫颈癌细胞存活及肿瘤移植模型肿瘤血管生成和肿瘤转移的影响

目的 分析来那度胺对人宫颈癌细胞存活及肿瘤移植模型肿瘤血管生成和肿瘤转移的影响。方法 采用CCK-8法检测体外空白组(加入二甲基亚砜)和实验组[加入不同剂量来那度胺(0. 01μmol/L、0. 10μmol/L、1. 00μmol/L、10. 00μmol/L、100. 00μmol/L)]对宫颈癌Si Ha细胞存活的影响。取20只C57BL/6小鼠,制备人宫颈癌Si Ha肿瘤移植的动物模型,随机分为空白组(于腹腔注射1%二甲基亚砜)和治疗组(于腹腔注射来那度胺50 mg/kg),每组各10只。造模21 d后,将两组小鼠处死,取其肿瘤组织。比较两组小鼠宫颈癌Si Ha细胞存活率、肿瘤重量和肿瘤体积的差异。行苏木精伊红染色,检测小鼠肿瘤组织内血管生成和肿瘤转移情况。结果 采用不同剂量来那度胺体外作用于宫颈癌Si Ha细胞2 d后,各组细胞吸光度值与空白组比较,无显著差异(P> 0. 05);吸光度值在不同剂量来那度胺的组间比较,也无显著差异(P> 0. 05);不同剂量来那度胺细胞生长抑制率的组间比较,无显著差异(P> 0. 05)。治疗组小鼠肿瘤重量和肿瘤体积较对照组均明显减少(P <0. 01)。相比对照组,治疗组小鼠肿瘤中心组织和边缘组织新生血管数量均显著减少(P <0. 01)。对照组小鼠肺组织内出现肿瘤转移灶,而治疗组小鼠未发现脑、肝、肺组织内肿瘤转移。结论 体外实验表明,应用不同浓度来那度胺对人宫颈癌Si Ha细胞存活并无明显影响;体内实验表明,来那度胺具有抑制小鼠Si Ha细胞抑制肿瘤生长的作用,可有效阻滞移植肿瘤组织内新生血管生成,并且在一定程度上具有阻滞肿瘤转移的功能。

Effects of lenalidomide on the survival of human cervical cancer cells and the angiogenesis and metastasis of tumors in tumor transplantation models

Objective To analyze the effects of lenalidomide on the survival of human cervical cancer cells and the angiogenesis and metastasis of tumors in transplantation models.Methods CCK-8 method was used to detect the effect of lenalidomide on the survival of SiHa cells from cervical cancer in blank group(add dimethyl sulfoxide)and experiment group(lenalidomide 0.01μmol/L,0.10μmol/L,1.00μmol/L,10.00μmol/L,100.00μmol/L)in vitro.Twenty C57BL/6 mice were randomly divided into control group(1%dimethyl sulfoxide was injected intraperitoneally)and treatment group(lenadomide was injected intraperitoneally at 50 mg/kg),10 mice in each group.After 21 days,the mice of the two groups were executed and their tumor tissues were taken.The survival rate,weight and volume of SiHa cells were compared between the two groups.Hematoxylin eosin staining was used to detect the angiogenesis and metastasis of tumors in mice.Results There was no significant difference in cell absorbance value between the two groups(P>0.05).There was no significant difference in cell absorbance value among subgroups of experiment group(P>0.05).There was no significant difference in cell growth inhibition rate between the two groups and among subgroups(P>0.05).The weight and volume of tumors in the treatment group were significantly lower than those in the control group(P<0.01).Compared with the control group,the number of neovascularization in the central and marginal tissues of tumors in the treatment group decreased significantly(P<0.01).Tumor metastasis was found in lung tissue of control group mice,but not in brain,liver and lung tissue of treatment group mice.Conclusion In vitro experiments showed that different concentrations of lenalidomide had no significant effect on the survival of human cervical cancer SiHa cells.In vivo experiments showed that lenalidomide could inhibit the growth of mouse SiHa cells,effectively block neovascularization in transplanted tumor tissues,and to some extent block swelling.Function of metastasis.