| 威灵仙类药材的薄层色谱鉴别研究 |
| |
| 引用本文： | 张玲玲,吴红梅,黄婕,靳凤云,王祥培.威灵仙类药材的薄层色谱鉴别研究[J].时珍国医国药,2020(1):111-113. |
| |
| 作者姓名： | 张玲玲 吴红梅 黄婕 靳凤云 王祥培 |
| |
| 作者单位： | 贵州中医药大学;贵州省药品检验所 |
| |
| 基金项目： | 贵州省一流学科建设项目([2017]008号);苗族医学研究协同创新中心(黔教合协同创新字[2015]05)。 |
| |
| 摘 要： | 目的建立威灵仙类药材的薄层色谱鉴别方法。方法以甲醇为溶剂超声提取,采用硅胶G薄层板,以三氯甲烷-丙酮(9∶1)、甲苯-乙酸乙酯-甲酸(20∶3∶0.2)、甲苯-丙酮-乙酸乙酯-甲酸(16∶4∶4∶0.25)、三氯甲烷-甲醇-水(9∶2∶2)为展开剂,对威灵仙类药材进行薄层定性鉴别研究。结果在365nm紫外灯下,威灵仙与混淆品的薄层色谱图有明显差异,在相同的色谱条件下,各样品的斑点颜色、位置、数量和Rf值均有差异,且斑点集中,分离效果好。结论该方法简便、易行、专属性强,可用于鉴别威灵仙类药材。
|
| 关 键 词： | 威灵仙类药材 薄层色谱法 定性鉴别 |
Study on Identification of Clematidis by TLC |
| |
| ZHANG Ling-ling,WU Hong-mei,HUANG Jie,JIN Feng-yun,WANG Xiang-pei.Study on Identification of Clematidis by TLC[J].Lishizhen Medicine and Materia Medica Research,2020(1):111-113. |
| |
| Authors: | ZHANG Ling-ling WU Hong-mei HUANG Jie JIN Feng-yun WANG Xiang-pei |
| |
| Institution: | (Guizhou University of Tradition Chinese Medicine,Guiyang 550025,China;Guizhou Pharmaceutical Inspection Institute,Guiyang 550002,China) |
| |
| Abstract: | Objective To establish the TLC identification method of Radix et Rhizoma Clematidis and its adulterants.Methods Ultrasonic extraction with methanol.Silica-G-CMC-Na plate was used with chloroform-acetone(9:1),toluene-ethyl acetate-Formicacid(20∶3∶0.2),toluene-acetone-Ethylacetate-Formicacid(14∶4∶4∶0.25),Chloroform-methanol-water(9∶2∶2)as developing solvent,Radix et Rhizoma Clematidis and its adulterants were identified by TLC.Results Under the 365nm UV,Radix et Rhizoma Clematidis and its adulterants thin layer chromatography there are significant differences.The spots color,position,quantity and Rf of each sample were different in the same chromatographic condition,and the spots were concentrated and the separation effect was good.Conclusion The method is simple and easy to operate,which can identify Clematidis. |
| |
| Keywords: | Clematidis TLC Qualitative identification |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
