MMP-9抑制剂对大鼠肝脏缺血再灌注模型的影响分析

目的探讨基质金属蛋白酶-9(MMP-9)抑制剂对大鼠肝脏缺血再灌注模型的影响。方法选择36只健康雄性Wistar大鼠,将其随机分成3组,对照组(大鼠未进行任何处理不建立肝脏缺血再灌注模型)12只,模型组(肝脏缺血再灌注模型组建立前采用生理盐水处理)12只大鼠,实验组(肝脏缺血再灌注模型建立前采用盐酸多西环素处理)12只。检测各组大鼠肝脏缺血再灌注1 h、6 h、24 h血清天冬氨酸氨基转移酶(AST)与丙氨酸氨基转移酶(ALT)含量;观察评价肝脏组织损伤情况,肝脏组织的淤血、空泡样变、坏死程度以及MMP-9水平。结果模型组与实验组再灌注1 h、6 h、24 h的血清ALT与AST水平、肝组织病理评分均显著高于对照组(P <0.05)实验组低于模型组(P <0.05),模型组和实验组在肝脏缺血再灌注6 h时血清ALT与AST水平最高(P <0.05)。模型组与实验组再灌注1 h、6h、24 h的肝组织MMP-9相对表达水平均显著低于对照组(P <0.05),实验组高于模型组(P <0.05)。结论 MMP-9抑制剂能抑制大鼠肝脏缺血再灌注损伤,促进恢复大鼠的肝功能,缓解肝组织病变状况。

Effect of MMP-9 inhibitor on hepatocellular ischemia reperfusion model in rats

Objective To investigate the effects of matrix metalloproteinase-9(MMP-9) inhibitor on hepatocellular ischemia reperfusion injury in rats.Methods 36 healthy male Wistar rats were randomly divided into 3 groups:control group(no treatment,n=12),hepatic ischemia-reperfusion model(treated with saline before hepatocellular ischemia reperfusion model,n=12),and model group(treated with doxycycline hydrochloride before hepatocellular ischemia reperfusion model,n=12).Serum AST and ALT levels were measured at 1 h,6 h and 24 h after hepatic ischemia reperfusion in rats of each group.Liver tissue injury,congestion,vascular degeneration,necrosis and MMP-9 levels were observed and evaluated.Results Serum ALT and AST levels and liver histopathological scores in the model group and the experimental group were significantly higher than those in the control group at 1 h,6 h,and 24 h after reperfusion(P <0.05).The experimental group were lower than the model group(P <0.05).Serum ALT and AST levels in model group and experimental group were the highest at 6 h of hepatic ischemia reperfusion(P <0.05).The relative expression levels of MMP-9 in the liver tissue of the model group and the experimental group at 1 h,6 h and 24 h after reperfusion were significantly lower than those in the control group(P <0.05).Those of the experimental group were higher than the model group(P <0.05).Conclusion MMP-9 inhibitor can inhibit hepatocellular ischemia reperfusion injury in rats,promote the recovery of liver function and alleviate the pathological changes of liver tissue.