金骨莲胶囊治疗大鼠类风湿性关节炎的作用及其机制研究

目的探讨金骨莲胶囊对大鼠类风湿性关节炎的治疗作用及相关机制。方法将60只大鼠随机分为6组,10只作为正常对照组,其余50只通过皮下注射Freund’s完全佐剂建立类风湿性关节炎大鼠模型。造模成功后随机分为五组,对照组不给于任何干预,另外四组分别给予塞来昔布、金骨莲胶囊(低剂量组,每次1粒,3次/d)、金骨莲胶囊(中剂量组,每次2粒,3次/d)以及金骨莲胶囊(高剂量组,每次3粒,3次/d)。观察各组大鼠给药后1 h与给药后7 d足关节肿胀度,给药后7 d血清白介素-17(IL-17)、白介素-23(IL-23)和肿瘤坏死因子-α(TNF-α)含量的变化;HE染色观察局部组织情况变化并应用关节组织病理学评分进行比较。结果给药后1 h与给药后7 d,模型组的足趾肿胀度高于正常对照组(P<0.05);与模型组比较,塞来昔布组、金骨莲胶囊组均可以明显减轻RA大鼠足关节肿胀度,差异有统计学意义(P<0.05),金骨莲胶囊组的作用呈剂量依赖性(P<0.05)。末次给药后7 d,与正常对照组比较,模型组大鼠血清IL-17、IL-35、TNF-α水平以及关节组织病理学评分均明显升高,差异有统计学意义(P<0.05)。与模型组比较,塞来昔布组以及金骨莲胶囊组的IL-17、IL-35、TNF-α以及关节组织病理学评分均明显降低,差异有统计学意义(P<0.05),金骨莲胶囊组的作用呈剂量依赖性(P<0.05)。结论金骨莲胶囊具有较好的治疗类风湿性关节炎作用,并且作用呈剂量依赖性,其作用机制可能与降低炎性因子的释放有关。

Effects of Jingulian capsule on the rheumatoid arthritis in rats and its mechanism

Objective To study the effects and mechanism of Jingulian Capsule on the rheumatoid arthritis in rats.Methods Sixty rats were randomly divided into 6 groups,10 for normal control group and the remaining 50 for establishing rheumatoid arthritis rat model by subcutaneous injection of Freund’s complete adjuvant.After successful modeling,they were randomly divided into five groups:model control group(with no intervention),celecoxib group,low dose Jinlinglian capsule group(1 capsule each time,3 times a day),medium dose Jinlinglian capsule group(2 capsules each time,3 times a day)and high dose Jingulian capsule group(3 capsules each time,3 times a day).The degree of swelling of the rat toe joints was observed at 1 h and 7 d after administration.Serum interleukin-17(IL-17),interleukin-23(IL-23)and tumor necrosis factor-αwere detected 7 days after administration.The changes of the local tissues were observed and compared by joint histopathological scores.Results At 1 h and 7 d after drug administration,the degree of toe joints swelling in the model groups were significantly higher than that in the normal control group(P<0.05).Compared with the model control group,the degree of toe joints swelling in the celecoxib group and the Jingulian capsule group were significantly relieved,in a dose-dependent manner for Jinlinglian capsules(P<0.05).At 7 days after the last administration,serum IL-17,IL-35,TNF-αlevels and joint histopathology scores in the model groups were significantly higher than those in the normal control group(P<0.05).Compared with the model control group,IL-17,IL-35,TNF-αand joint histopathology scores in the celecoxib group and the Jingulian capsule groups were significantly lower(P<0.05),in a dose-dependent manner for Jinlinglian capsules(P<0.05).Conclusion Jingulian Capsule has a good therapeutic effect on rheumatoid arthritis in a dose-dependent manner.The mechanism may be related to the reduction of inflammatory cytokines release.