丹红注射液对脑缺血再灌注损伤大鼠皮质区新生微血管的影响研究

背景新生血管形成是最终的侧支代偿途径,探究丹红注射液对新生血管的影响对其作用机制研究具有一定价值。目的探究丹红注射液对脑缺血再灌注损伤大鼠皮质区新生微血管的影响。方法2018年12月—2019年6月,将84只雄性SD大鼠随机分为假手术组(n=12)、造模组(n=36)及丹红组(n=36)。参照改良LONGA线栓法制备脑缺血再灌注损伤大鼠模型,其中造模组和丹红组大鼠制备脑缺血再灌注损伤模型,假手术组大鼠不插线栓;丹红组大鼠于拔出线栓后腹腔注射丹红注射液,造模组和假手术组大鼠均于相同时间点腹腔注射等量0.9%氯化钠溶液。比较三组大鼠造模后24 h、72 h、7 d神经行为学评分、脑梗死体积及脑缺血再灌注皮质区新生微血管数目。结果(1)假手术组大鼠造模后24 h、72 h及7 d神经行为学评分均为0。造模组和丹红组大鼠造模后24 h、72 h神经行为学评分比较,差异无统计学意义(P>0.05);丹红组大鼠造模后7 d神经行为学评分低于造模组(P<0.05)。(2)假手术组大鼠造模后24 h、72 h及7 d脑梗死体积均为0。丹红组大鼠造模后24 h、72 h及7 d脑梗死体积小于造模组(P<0.05)。(3)造模组和丹红组大鼠造模后24 h脑缺血再灌注皮质区新生微血管数目多于假手术组(P<0.05);造模组和丹红组大鼠造模后72 h及7 d脑缺血再灌注皮质区新生微血管数目多于假手术组,丹红组大鼠脑缺血再灌注皮质区新生微血管数目多于造模组(P<0.05)。结论丹红注射液可有效增加脑缺血再灌注损伤大鼠皮质区新生微血管数量,改善脑侧支循环,进而减轻神经功能缺损、缩小脑梗死体积。

Impact of Danhong Injection on Neovascularization in Cortex in Rats with Cerebral Ischemia-reperfusion Injury

Background Neovascularization is the ultimate way of collateral compensation,thus it is with reference value to explore the impact and action mechanism of Danhong injection on neovascularization.Objective To investigate the impact of Danhong injection on neovascularization in cortex in rats with cerebral ischemia-reperfusion injury.Methods From December 2018 to June 2019,a total of 84 male SD rats were randomly divided into sham-operation group(n=12),model group(n=36)and Danhong group(n=36).Model of cerebral ischemia-reperfusion injury was established by modified LONGA suture-occluded method,thereinto rats in model group and Danhong group were prepared for model of cerebral ischemia-reperfusion injury,while rats in sham-operation group did not receive suture-occlusion;rats in Danhong group received intraperitoneal injection of Danhong injection after suture-occlusion,while rats in model group and sham-operation group received intraperitoneal injection of same amount of 0.9%sodium chloride solution after suture-occlusion.Neurobehavioral score,volume of cerebral infarction and number of neovascularization in cerebral ischemia-reperfusion cortex were compared in the three groups 24 hours,72 hours and 7 days after modeling.Results(1)Neurobehavioral score in sham-operation group were all 024 hours,72 hours and 7 days after modeling.There was no statistically significant difference in neurobehavioral score between model group and Danhong group 24 or 72 hours after modeling(P>0.05),while neurobehavioral score in Danhong group was statistically significantly lower than that in model group 7 days after modeling(P<0.05).(2)Volume of cerebral infarction in sham-operation group were all 024 hours,72 hours and 7 days after modeling.Volume of cerebral infarction in Danhong group was statistically significantly smaller than that in model group 24 hours,72 hours and 7 days after modeling,respectively(P<0.05).(3)Number of neovascularization in cerebral ischemia-reperfusion cortex in model group and Danhong group was statistically significantly more than that in sham-operation group 24 hours after modeling,respectively(P<0.05);number of neovascularization in cerebral ischemia-reperfusion cortex in model group and Danhong group was statistically significantly more than that in sham-operation group 72 hours and 7 days after modeling,respectively,meanwhile number of neovascularization in cerebral ischemia-reperfusion cortex in Danhong group was statistically significantly more than that in model group,respectively(P<0.05).Conclusion Danhong injection can effectively increase the number of neovascularization in cortex in rats with cerebral ischemia-reperfusion injury,improve the collateral circulation of brain,and then reduce the neurological deficit and volume of cerebral infarction.