中国北方汉族人2型糖尿病合并冠心病与KIF6基因Trp719Arg多态性的相关性

目的探讨中国北方汉族人群KIF6基因Trp719Arg多态性基因型和等位基因频率分布特点,及与2型糖尿病(DM)合并冠心病(CHD)的关联性。方法采用病例对照研究设计,应用聚合酶链反应限制性片段长度多态性(PCR-RFLP)技术分析了对照组(152例)、DM组(97例)、DM+CHD组(76例)KIF6基因Trp719Arg多态性;比较组间基因型和等位基因频率分布差异,研究基因多态性对血糖、血脂水平的影响。结果中国北方汉族人群Trp719Arg多态性TT、TC、CC基因型频率分别为0.281、0.499与0.220。T、C等位基因频率分别为0.531与0.469。KIF6基因Trp719Arg多态性基因型和等位基因频率组间分布差异无统计学意义(均为P>0.05)。KIF6基因Trp719Arg多态性对血糖、血脂水平无显著影响。Logistic回归分析显示,高血压、年龄(≥60岁)、低HDL-C水平(<1.04 mmol/L)是DM+CHD的独立危险因素(OR分别为2.850、12.977和4.006,均为P<0.05),C等位基因与DM+CHD的发生无统计学相关性。结论 KIF6基因Trp719Arg多态性可能不是我国北方汉族人群DM+CHD的独立危险因素。

Association between KIF6 Trp719Arg polymorphism and type 2 diabetes mellitus with coronary heart disease in Han Nationality from northern China

Objective To explore kinesis-like protein 6(KIF6) gene Trp719Arg polymorphism and its association with type 2 diabetes mellitus(T2DM) combined with coronary heart disease(CHD) in Han nationality from northern China. Methods A total of 325 subjects including 152 controls,97 T2DM patients,and 76 patients with T2 DM combined with CHD were enrolled in this case-control study.KIF6 Trp719Arg polymorphism was analyzed by using polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).The relationships between KIF6 gene polymorphism and hemoglobinA1c(HbA1c),serum lipids,and fasting blood glucose(FBG) were also studied. Results For the Han Chinese,the genotype frequencies of TT,TC,CC were 0.281,0.499 and 0.220,respectively.The allele frequencies of T and C were 0.531 and 0.469.Neither the frequencies of genotypes nor those of alleles of KIF6 Trp719Arg polymorphisms were statistically different among these groups(all P>0.05).Serum levels of HbAlc,lipids and FBG were not correlated with KIF6 gene variation.Logistic regression analysis showed that history of hypertension,age and lower HDL-C(<1.04 mmol/L) were risk factors for T2DM with CHD(OR=2.850,12.977 and 4.006,respectively,all P<0.05).C allele was not associated with T2DM with CHD(OR=0.694,P=0.193,95%CI=0.400-1.204). Conclusions KIF6 Trp719Arg polymorphism is not a risk factor for patients with T2DM and CHD in Han nationality from northern China.