儿童血清脂肪细胞因子与肥胖和非酒精性脂肪肝的关联

目的 研究白细胞介素-6(IL -6)、血浆纤溶酶原激活物抑制剂1(PAI -1)、视黄醇结合蛋白4(RBP -4)水平在非酒精性脂肪肝(NAFLD)、肥胖儿童血清中的变化,为儿童NAFLD血清学标志物的发现提供依据.方法 采用双抗体夹心酶联免疫吸附法,测定儿童血清IL -6、PAI -1、RBP -4水平.结果 体重正常组与肥胖组IL -6、PAI -1水平差异均无统计学意义(P值均＞0.05),体重正常组RBP -4水平显著高于肥胖组(P＜0.01)；NAFLD组的IL -6水平明显高于非NALFD组(P＜0.01),NAFLD组的RBP -4水平显著低于非NAFLD(P＜0.01)；未见NAFLD组与非NAFLD组之间PAI -1水平存在差异(P＞0.05).NAFLD分度与IL -6水平呈正相关(β=0.168,P=0.013),与RBP-4呈负相关(β=- 0.262,P=0.001).结论 肥胖儿童RBP-4水平低于正常体重儿童；IL -6及RBP -4与儿童NAFLD有关,可能在儿童脂肪肝的发病过程中起一定作用.

Association between serum IL-6,PAI-1,RBP-4 levels and obesity,nonalcoholic fatty liver disease in children

Objective To study the relationship between serum IL-6,PAI-1 and RBP-4 levels and obesity,nonalcoholic fatty liver disease in children.Methods The quantitative enzyme linked sandwich immunoassay(ELISA) was used to detect the serum concentrations of IL-6(n=217),PAI-1(n=213) and RBP-4(n=157).Results No significant differences of IL-6 or PAI-1 levels were found between obesity and normal weight children(P=0.44 and P=0.40,respectively),while normal weight children had higher RBP-4 level than obese children(P<0.01).Children with NAFLD had higher IL-6 but lower RBP-4 level compared to non-NAFLD children(P<0.01).No significant difference of PAI-1 level was found between NAFLD and non-NAFLD children(P>0.05).NAFLD degrees were positively related with IL-6 level and negatively related with RBP-4(β=0.168,P=0.013;β=-0.262,P=0.001,respectively).Conclusion RBP-4 may play a role in childhood obesity;IL-6 and RBP-4 are associated with NAFLD in children and may involve in pathological process in childhood NAFLD.