| 丙型肝炎病毒核心蛋白反式激活基因2表达产物的亚细胞定位 |
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| 引用本文： | 王建军,赵平,靳雪源,成军,卿松. 丙型肝炎病毒核心蛋白反式激活基因2表达产物的亚细胞定位[J]. 中华实验和临床感染病杂志(电子版), 2010, 4(1): 7-8 |
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| 作者姓名： | 王建军 赵平 靳雪源 成军 卿松 |
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| 作者单位： | 1. 解放军第302医院,北京市,100039
2. 首都医科大学北京地坛医院传染病研究所 |
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| 摘 要： | 目的研究HCV核心蛋白反式激活基因2(TAHCCP2)在细胞内的表达及表达产物的亚细胞定位。方法构建HBeAgTP基因的绿色荧光蛋白表达质粒pEGFP-C1-TAHCCP2,转染HepG2细胞,24h后荧光显微镜下观察表达蛋白的亚细胞定位。结果成功构建出TAHCCP2基因的绿色荧光蛋白表达质粒pEGFP-C1-TAHCCP2,其表达的蛋白定位于细胞核。结论TAHCCP2基因可表达TAHCCP2蛋白且定位于细胞核。
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| 关 键 词： | 丙型肝炎病毒 TAHCCP2蛋白 |
Subcellular location of production of gene 2 transactivated by HCV core protein |
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| WANG Jian-jun,ZHAO Ping,JIN Xue-yuan,CHENG Jun,QING Song. Subcellular location of production of gene 2 transactivated by HCV core protein[J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Version), 2010, 4(1): 7-8 |
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| Authors: | WANG Jian-jun ZHAO Ping JIN Xue-yuan CHENG Jun QING Song |
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| Affiliation: | ,The 302 Hospital of PLA,Beijing 100039,China |
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| Abstract: | Objective To study the subcellular location of gene 2 transactivated by HCV core protein （TAHCCP2）.Methods Green fluorescent protein （GFP） expression vector pEGFP-TAHCCP2 was established and transfected into HepG2 cells and the subcellular location of the proteins expressed by TAHCCP2 were analyzed through green fluorescent microscopy after 24 hours.Results The pEGFP-TAHCCP2 vector was successfully cloned,TAHCCP2 protein was expressed in cells and subcellularly located in nuclei.Conclusions TAHCCP2 gene can express TAHCCP2 protein which locates subcellularly in nuclei. |
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| Keywords: | Hepatitis C virus TAHCCP2 protein |
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