| 山东肿足蕨总三萜的提取工艺优化研究 |
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| 引用本文： | 张华丽,朱军,高桂花,张敏娜,李彦连. 山东肿足蕨总三萜的提取工艺优化研究[J]. 中药材, 2012, 0(4): 648-650 |
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| 作者姓名： | 张华丽 朱军 高桂花 张敏娜 李彦连 |
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| 作者单位： | 济宁医学院药学院;山东济宁学院生命科学与工程系 |
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| 基金项目： | 济宁医学院青年科研基金重点项目(JYQN) |
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| 摘 要： | 目的:优化山东肿足蕨总三萜提取工艺。方法:以5%香草醛-冰醋酸溶液和72%浓硫酸为显色系统,总三萜含量为考察指标,采用单因素和正交试验优选山东肿足蕨总三萜的提取工艺。结果:确定用12倍量60%乙醇为溶剂,在60℃条件下超声提取60 min为最佳提取条件。结论:优选的工艺稳定可行,可作为山东肿足蕨总三萜的提取工艺。
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| 关 键 词： | 山东肿足蕨 总三萜 正交试验 |
Optimum Extraction Technology of Total Triterpenoids from Hypodematium sinense |
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| ZHANG Hua-li,ZHU Jun,GAO Gui-hua,ZHANG Min-na,LI Yan-lian. Optimum Extraction Technology of Total Triterpenoids from Hypodematium sinense[J]. Journal of Chinese medicinal materials, 2012, 0(4): 648-650 |
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| Authors: | ZHANG Hua-li ZHU Jun GAO Gui-hua ZHANG Min-na LI Yan-lian |
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| Affiliation: | 1.School of Pharmacy,Jining Medical University,Rizhao 276826,China;2.Life Science and Engineering Department,Jining University,Qufu 273155,China) |
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| Abstract: | Objective:To optimize the extraction technology of total triterpenoids from Hypodematium sinense.Methods:With 5% vanillin-glacial acetic acid solution and 72% sulfuric acid as chromogenic agent and the content of total tripenoids as index,using single factor experiment and orthogonal test,the optimal extraction condition was determined.Results:The optimal conditions were solid-liquid ratio 1∶ 12,60% ethanol concentration,and ultrasonic extraction time of 60 min at 60 ℃.Conclusion:The extraction technology is feasible and can be used as extraction process of total triterpenoids from Hypodematium sinense. |
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| Keywords: | Hypodematium sinense Iwatsuri Totals triterpenoids Orthogonal text |
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