Total saponins of Panax Notoginseng modulate the expression of caspases and attenuate apoptosis in rats following focal cerebral ischemia-reperfusion |
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| Authors: | Hua Li Chang-Qing Deng Bei-Yang Chen Shu-Ping Zhang Yan Liang Xue-Gang Luo |
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| Institution: | 1. Laboratory of Pathophysiology, Hunan University of Traditional Chinese Medicine, Changsha, Hunan Province, PR China;2. Department of Histology and Embryology, Hunan University of Traditional Chinese Medicine, Changsha, Hunan Province, PR China;3. Department of Anatomy and Neurobiology, Xiangya School of Medicine, Central South University, Changsha, Hunan Province, PR China |
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| Abstract: | Ethnopharmacological relevanceTotal saponins of Panax notoginseng (TSPN), main constituents extracted from Panax Notoginseng, a highly valued traditional Chinese medicine, have been shown to be an effective agent on cerebral infarction.Aim of the studyThe effects of TSPN on apoptosis and expressions of caspase-1, caspase-3 and caspase-8 were studied after cerebral ischemia for 2 h followed by reperfusion for 46 h in rats.Materials and methodsRats were subjected to transient middle cerebral artery occlusion (MCAO) model using the intraluminal thread. TSPN was administered intraperitoneally at 5 min before and 12 h, 24 h and 36 h after MCAO, respectively.ResultsTSPN (at the dose of 25 mg/kg) significantly attenuated TUNEL-positive cells and reduced the expression of caspase-1 and caspase-3 compared to the model group, while it had no obvious effect on the expression of caspase-8.ConclusionsThe neuroprotective effect of TSPN on focal ischemia may be related to inhibition of apoptosis and caspases activation. |
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| Keywords: | Total saponins of Panax notoginseng Cerebral ischemia-reperfusion TUNEL Caspase-1 Caspase-3 Caspase-8 |
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