Anti-inflammatory activities of Sieboldogenin from Smilax china Linn.: Experimental and computational studies |
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Authors: | Inamullah Khan Muhammad Nisar Farooq Ebad Said Nadeem Muhammad Saeed Haroon Khan Samiullah Fazli Khuda Nasiara Karim Zia Ahmad |
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Affiliation: | 1. Department of Pharmacy, University of Peshawar, Peshawar 25120, Pakistan;2. Institute of Chemical Sciences, University of Peshawar, Peshawar 25120, Pakistan;3. H.E.J. Research Institute of Chemistry, International Center for Chemical Sciences, University of Karachi, Karachi, Pakistan |
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Abstract: | Ethnopharmacological relevanceSmilax china Linn. is extensively used in traditional Chinese medicine (TCM) as well as in Pakistan for several medicinal purposes including their use in inflammatory disorders.Aim of the studyThe aims of the current study were to validate and assess the folk use of Smilax china Linn. on pharmacological grounds using the isolated compound at molecular, in vivo and computational levels.Materials and methodsSeiboldogenin was isolated from ethyl acetate fraction of the plant crude extract. In vitro lipoxygenase and in vivo carrageenan-induced hind paw oedema models were used in experimental studies while molecular docking technique was used to conduct computational study.ResultsSieboldogenin showed significant lipoxygenase inhibition (IC50: 38 μM). It also exhibited significant inhibition (p < 0.05) of carrageenan-induced hind paw oedema at the doses of 10 and 50 mg/kg. Computational molecular docking showed its molecular interaction with important amino acid residues in the catalytic site of lipoxygenase, revealing its potential binding mode at molecular level.ConclusionsSieboldogenin seems to be a potential new anti-inflammatory compound responsible for anti-inflammatory activities of Smilax china Linn. Its in vitro and in vivo inflammatory activities are in good agreement with the folk medicinal use of Smilax china Linn. in inflammatory disorders. |
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Keywords: | Smilax china Linn. Lipoxygenase Anti-inflammatory Sieboldogenin Molecular docking |
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