| 选择性牛眼上皮样小梁细胞的体外培养 |
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| 引用本文： | 张文强,杨新光,李养军. 选择性牛眼上皮样小梁细胞的体外培养[J]. 眼科新进展, 2005, 25(6): 510-512 |
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| 作者姓名： | 张文强 杨新光 李养军 |
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| 作者单位： | 1. 430070,湖北省武汉市,广州军区武汉总医院眼科
2. 710038,陕西省西安市,第四军医大学唐都医院眼科 |
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| 摘 要： | 目的选择性行牛眼上皮样小梁细胞的体外培养，为进一步研究原发性开角型青光眼提供实验材料。方法利用组织块法进行小梁细胞的原代培养，之后利用机械划除法、酶消化法、反复贴壁法，对牛眼上皮样小梁细胞进行选择性培养和传代。结果成功选择性培养出形态、性状良好的牛眼上皮样小梁细胞。结论选择性牛眼上皮样小梁细胞的体外培养所获细胞形态单一，可以用此种方法进行牛小梁细胞的体外培养并为实验应用做好准备。
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| 关 键 词： | 小梁细胞 牛眼 上皮样细胞 培养 |
| 文章编号： | 1003-5141(2005)06-0510-03 |
| 收稿时间： | 2004-07-05 |
| 修稿时间： | 2004-08-10 |
Epithelial-like bovine trabecular meshwork cells selective cultured in vitro |
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| ZHANG Wen-Qiang,YANG Xin-Guang,LI Yang-Jun. Epithelial-like bovine trabecular meshwork cells selective cultured in vitro[J]. Recent Advances in Ophthalmology, 2005, 25(6): 510-512 |
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| Authors: | ZHANG Wen-Qiang YANG Xin-Guang LI Yang-Jun |
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| Abstract: | Objective To explore the selective cultured method of epithelial-like bovine trabecular meshwork cells in vitro, in order to provide the experimental materials for the study on primary open angle glaucoma.Methods Tissue method was used to obtain primary bovine trabecular meshwork cells, then machtntcal removing method, enzyme digestion method and repeatedly anchor method were used to obtain epithelial-like bovine trabecular meshwork cells. Results Success to get cultured epithelial-like bovine trabecular meshwork cells.Conclusion Epithelial-like bovine trabecular meshwork cells with single shapes and stable characteristics could be obtained by selective culture method in vitro. |
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| Keywords: | trabecular meshwork cell bovine eye epithelial-like cell cultured |
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