轻度认知障碍患者血小板中α、β-分泌酶基因表达水平及其临床意义

目的探讨轻度认知障碍患者血小板中α、β-分泌酶基因表达水平及其临床意义。方法选入28例轻度认知障碍(MCI)患者,以28例老年性痴呆(AD)及21例正常老人为对照,分别用实时荧光定量PCR检测各组血小板中的ADAM10、BACE1基因表达水平,用ELISA法检测各组血浆中Aβ42含量。结果 3组中β-分泌酶(BACE1)基因表达水平有明显差异(P<0.05),AD组、MCI组的表达水平是正常老人的4.51倍、2.22倍。α分泌酶基因表达水平及Aβ42含量无明显差异(P>0.05),相关分析显示Aβ42含量与α、β-分泌酶基因表达水平无关(r=-0.1442,r=0.1611,P均>0.05)。结论 MCI患者血小板中的BACE1的基因表达上调可能与MCI的转向(向AD转化)有关。MCI患者应尽早接受相关的酶学检测并实施干预。

Expression of platelet ADAM10 and BACE1 mRNA and its clinical significance in mild cognitive impairment

Objective To detect the expression of ADAM10 and BACE1 mRNA in mild cognitive impairment and explore the roles of ADAM10 and BACE1 in the development of MCI.Methods 28 cases with MCI,28 cases with AD and 21 cases normal elders were selected.Real-time fluorescence quantitative RT-PCR was performed for detecting the expression of ADAM10 and BACE1 mRNA,A sandwich ELISA was performed for detecting the blood serum Aβ42 concentration.Results The FQ-PCR results showed that the expression level of Platelet BACE1 mRNA in AD,MCI groups were 4.51-fold and 2.22-fold higher than the normal elders respectively(P<0.05).The expression level of Platelet ADAM10 mRNA and serum Aβ42 concentration showed no differences(r=-0.1442,r=0.1611,P>0.05).Serum Aβ42 concentration showed no correlations with expression levels of Platelet ADAM10 BACE1 mRNA respectively.Conclusions Platelet BACE1 mRNA expression might be associated with the development MCI and may potentially used as an indicator for predicting the prognosis of MCI.