癫痫发作大鼠海马神经元凋亡与caspase-3 mRNA表达的研究

目的 :研究癫大鼠海马神经元凋亡与caspase 3mRNA表达的关系。方法 :采用大鼠红藻氨酸 (KA)致模型 ,以原位末端标记 (TUNEL)检测癫后不同时间海马神经元凋亡 ;RT PCR检测caspase 3mRNA的表达。结果 :KA致后 1d ,海马CA1、CA3及CA4区开始出现凋亡细胞 ,3d时明显增多 ,7d时最多。KA致后 6h ,海马组织caspase 3mRNA表达显著增高 ,1、3、7d仍持续高水平表达。结论 :癫大鼠海马神经元凋亡与caspase 3mRNA的表达密切相关 ,caspase 3在神经元凋亡过程中起着重要的作用

Neuronal Apoptosis and Caspase-3 Mrna Expression in Rat Hippocampus Following Kainic Acid-evoked Epileptic Seizures

Aim:To explore the relationship between neuronal apoptosis and caspase-3 mRNA expression in rat hippocampus following epileptic seizures.Methods:With a rat model of kainic acid-evoked epileptic seizures,apoptosis of hippocampal neurons was determined by terminal deoxynucleotidyl transferase-mediated dUTP-biotin in situ nick end labeling(TUNEL) at different times following epileptic seizures.Meanwhile,caspase-3 mRNA was determined by semiquantitative transcripition-polymerase chain reaction(RT-PCR).Results:1d after epileptic seizures,apoptotic neurons were detected in CA 1,CA 3 and CA 4 subfields.The number of apoptotic neurons increased significantly at 3d and reached the most at 7d after epileptic seizures.At 6h after epileptic seizures,the expression of caspase-3 mRNA in rat hippocampus increased significantly and remained at high levels at 1,3,7 d.Conclusion:There was a high correlation between neuronal apoptosis and caspase-3 mRNA expression in rat hippocampus after epileptic seizures,The result suggests that caspase-3 play an important role in hippocampal neuronal apoptosis after epileptic seizures.