| RP-HPLC测定天然与人工冬虫夏草中游离麦角甾醇的含量 |
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| 引用本文： | 李绍平, 李萍, 季晖, 张平. RP-HPLC测定天然与人工冬虫夏草中游离麦角甾醇的含量[J]. 中国现代应用药学, 2001, (4): 297-299. |
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| 作者姓名： | 李绍平 李萍 季晖 张平 |
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| 作者单位： | 1. 中国药科大学南京 210009
2. 安徽医科大学附属医院药剂科合肥 230022 |
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| 摘 要： | 目的：比较天然与人工冬虫牙草中游离麦角甾醇的含量，探讨麦角甾醇作为冬虫夏草质控指标的可行性。方法：采用HP1100高效液相色谱仪，Allsphere C18色谱柱，甲醇－水（95∶5）为流动相，检测波长为275nm，流速1ml/min。结果：不同来源的天然冬虫夏草游离麦角甾醇含量差异显著，但均明显高于人工虫草菌丝。结论：麦象甾醇可作为冬虫夏草质控指标之一。
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| 关 键 词： | RP-HPLC 冬虫夏草 麦角甾醇 |
RP-HPLC Determination of ergosterol in natural and cultured Cordyceps |
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| Shaoping Li, Ping Li, Hui Ji, . RP-HPLC Determination of ergosterol in natural and cultured Cordyceps[J]. Chinese Journal of Modern Applied Pharmacy, 2001, (4): 297-299. |
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| Authors: | Shaoping Li Ping Li Hui Ji ( |
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| Abstract: | OBJECTIVE:A method for the determination of free ergosterol in natural and cultured Cordyceps by RP HPLC is described. METHOD:C18 column was used with methanol water (95: 5) as mobile phase. The flow rate was 1 ml/min and detected at 275nm.RESULTS:The average recovery was 99.7% and relative standard deviation was 1.5% (n=3).CONCLUSION:This method is simple, rapid and sensitive. |
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| Keywords: | RP HPLC Cordyceps ergosterol |
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