补肾调冲方含药血清对高雄激素培养大鼠卵巢颗粒细胞增殖与分泌的影响

目的 运用血清药理学方法观察补肾调冲方对高雄激素培养的大鼠卵巢颗粒细胞增殖与分泌功能的影响。方法 根据血清药理学方法，用6周龄的未成年SD大鼠制备促卵泡生成素（FSH）及大、中、小剂量补肾调冲方含药血清，分别加入过量丙酸睾丸酮处理的颗粒细胞培养体系中。培养48h后，测定^3H-TdR掺入DNA的量，并用流式细胞仪检测细胞周期分布及增殖指数（PI），放免法测定颗粒细胞培养液中雌二醇（E2）、孕酮（P）水平及细胞内环磷酸腺苷（cAMP）含量。结果 高雄激素明显抑制颗粒细胞的增殖与分泌功能，并明显降低了细胞内cAMP的含量；而补肾调7中方含药血清可改善高雄激素对颗粒细胞功能的抑制状态，促进细胞增殖及E2、P的分泌，其作用呈剂量依赖性。结论 补肾调冲方含药血清可有效拮抗高雄激素对颗粒细胞功能的抑制作用，推测补肾调冲方通过促卵泡发育、提高雌、孕激素水平，改善雌／雄激素比值来调节卵巢功能。

Effects of Bushen Tiaochong Recipe Contained Serum on Proliferation and Secretion of Ovarian Granulocyte Cultured with Excessive Androgen

ObjectiveTo observe the effect of Bushen Tiaochong Recipe (BTR) on proliferation and secretion of ovarian granulocyte (OGC) cultured with excessive androgen by serum pharmacologic technique. MethodsFollicular stimulating hormone (FSH) contained and BTR contained (high, moderate or low dose) serum prepared on 6-week-old Sprague Dawley rats by serum pharmacologic method were respectively added into cultured OGC cell-line after treatment with testosterone propionat (TP). Then the cells were incubated for 48 hrs. The quantity of 3H-thymidine incorporated DNA was determined with a scintillation counter; the cell cycle distribution and proliferation index (PI) were estimated with flow cytometer (FCM); and the contents of estrodial and progestogen in culture fluid and intracellular cAMP were detected by radioimmuoassay. ResultsExcessive androgen obviously inhibited the proliferation and secretion of OGC, and reduced the intracellular cAMP. BTR contained serum could improve above-mentioned inhibition of androgen to promote the cell proliferation and secretion of E_2 and P in dose-dependant manner. Conclusion BTR could alleviate the inhibitory action of excessive androgen on OGC, the ovarian function may be adjusted by BTR through promoting follicular development, increasing the levels of estrogen and progestogen, improving the ratio of estrogen/androgen.