穿破石对四氯化碳所致小鼠急性肝损伤的保护作用

目的 研究穿破石对四氯化碳( CCl4)所致小鼠急性肝损伤的保护作用,探讨其疗效机制.方法 采用急性四氯化碳肝损伤模型,给予小鼠穿破石水提取物和醇提取物灌胃8d,观察其对给予0.1％四氯化碳16h后检测小鼠血清中谷丙转氨酶(ALT)和谷草转氨酶(AST)活性；同时测定肝组织中丙二醛(MDA)、超氧化物歧化酶(SOD)和总蛋白(TP)含量.结果 与四氯化碳模型组比较,穿破石水提取物和醇提取物可使血清ALT、AST活性明显降低(P＜0.05)；同时穿破石水提取物和醇提取物可使肝组织中MDA的含量明显下降(P＜0.05),SOD含量明显增强(P＜0.05),对TP的含量变化无影响(P＞0.05).结论 穿破石对四氯化碳所致小鼠急性肝损伤具有保护作用,作用机制可能与其抗脂质过氧化有关.

Protective Effect of Cudrania cochin chinensis against Experimental Acute Liver Injury Induced by Carbon Tetrachloride

Objective To study the protective effects and mechanism of Cudrania cochin chinensis against experimental acute liver injury in mice induced by carbon tetrachloride(CCL4).Methods The liver-injury model was established in mice by carbon tetrachloride.Mice were administered with water and alcohol extract of Cudrania cochin chinensis for 8 days,then the liver injury was induced by intraperitoneal(ip) injection of carbon tetrachloride(10ml/kg).After 16 hours,the serum contents of ALT,AST and the contents of SOD,MDA and TP in liver were detected.Results Compared with the model group,the contents of ALT,AST in serum were obviously decreased(P<0.05),the content of MDA in liver was also lowered(P<0.05),and the activity of SOD in liver was markedly improved(P<0.05) after preventive treatment with water and alcohol extract of Cudrania cochin chinensis.Conclusion Cudrania cochin chinensis has protective effects against acute liver injury induced by carbon tetrachloride in mice and the mechanism may be related to its anti-lipid peroxidation.