普罗布考对高脂家兔胸主动脉内皮储备功能的保护作用

为观察口服普罗布考对高胆固醇血症和动脉粥样硬化免主动脉的内皮储备功能的影响,并探讨其可能作用机制。将26只新西兰白兔(体重1.5-2.5 kg)随机分为3组:正常饮食组(普通饮食,n=6)、高脂饮食组(高脂饮食,n=10)、普罗布考治疗组(高脂饮食+普罗布考200mg/kg,n=10)。在高脂饲养前和饲养后第12周,抽血测血清总胆固醇浓度。第12周后处死动物,取胸主动脉,检测离体胸主动脉环对乙酰胆碱与硝普钠的反应。结果发现,①普罗布考有降血脂作用(39.1％);②与正常饮食组相比,高脂血症兔胸主动脉对乙酰胆碱引起的最大的内皮依赖性舒张反应明显降低(0.65±0.14比0.16±0.04,P<0.05),普罗布考能明显保护高胆固醇血症所损伤的内皮依赖性舒张反应(两组最大血管舒张度分别为0.60±0.10比0.16±0.04,P<0.05);③3组对硝普钠所致的非内皮依赖性舒张反应均没有差别。结果提示普罗布考主要是通过降血脂和抗氧化两个途径来保护高脂家兔胸主动脉内皮储备功能。

Protection of probucol on endothelial dysfunction of HCHF-diet rabbits

Aim To observe the effect of probucol on endothelium-dependent vasodilator activity in high cholesterol plus high fat diet (HCHF) rabbits. Methods 26 New Zealand white rabbits (1.5-2.5 kg) were randomly divided into three groups, that is, control group ( n = 6, normal diet); hyperlipidemic group ( n = 10, HCHF-diet); probucol group ( n = 10, HCHF-diet plus probucol 200 mg/kg everyday). There blood was drawn from the central ear artery to analyze plasma total cholesterol (TC) at the pre-experiment and in the 12th week of post experiment. Then all rabbits were killed in the 12 th week and thoracic aortas were isolated to analyze vascular endothelial function. Results Probucol could remarkably lower plasma TC lever,compared to hyperlipidemic group, TC level decreased 39.1 % in probucol group ( P < 0.01). The HCHF diet significantly attenuated endothelium-dependent relaxation (EDR) of rabbit aortic rings to acetylcholine (Ach), but probucol could significantly protected against HCHF diet-injured EDR of rabbit aortic rings. The HCHF diet and probucol did not significantly influence the endothelium-independent relaxation of the rabbit artery elicited by sodium nitroprusside (SNP). Conclusion Probucol can protect against injuries of vascular endothelial function induced by hypercholesterolemia through lowering plasma TC and anti-oxidant.