维吾尔族、汉族冠心病人群CYP2C19基因多态性分析

目的：探讨细胞色素P4502C19（CYP2C19）在维吾尔族、汉族冠心病人群中的基因型及等位基因分布特征，并考察年龄对其基因多态性的影响。方法：选择355例（维族194例、汉族161例）无亲缘关系的冠心病患者，采用多聚酶链反应-限制性片段长度多态性（PCR-RFLP）法分析维、汉族 CYP2C19基因特征，并比较两族不同年龄组（＜60岁和≥60岁）基因多态性的差异。结果：与汉族患者比较，维族患者等位基因＊1显著增加[（64.9％比75.2％），P＜0.01]，等位基因＊2（29.5％比22.2％）和等位基因＊3（5.6％比2.6％）频率显著降低（P均＜0.05），；基因型＊1/＊1显著增加[（38.5％比57.7％），P＜0.01]，＊1/＊2显著降低[（44.7％比31.5％），P＜0.05]，其他4基因型两族无显著差异（P＞0.05）；快代谢型频率（38.5％比57.7％）显著增加，中代谢型（52.8％比31.5％）显著降低（P均＜0.01）；同一民族中，年龄对其基因型及等位基因分布差异无显著影响（P均＞0.05）。结论：维、汉族冠心病患者细胞色素 P4502C19基因型及等位基因分布有显著不同，了解其特征对指导临床用药，改善患者预后具有重要意义。

Genetic polymorphism analysis of CYP2C19 in Uighur and Han nationality patients with coronary heart ;disease

Objective：To explore the genotype and allele distribution feature of cytochrome P450 2C19 （CYP2C19） in Uighur and Han nationality patients With coronary heart disease （CHD）.Methods：A total of 355 CHD patients Without kinship,including 194 Uighur cases and 161 Han cases,Were selected. CYP2C19 genetic polymorphism Was analyzed using polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）,it’ s compared betWeen different age groups （〈60 years and≥60 years）of the tWo nationalities.Results：Compared With Han na-tionality patients,there Were significant increase in allele＊1 frequency [（64.9% vs.75.2%）,P〈0.01],signifi-cant decrease in allele＊2 （29.5% vs.22.2%）,and allele＊3 （5.6% vs.2.6%） frequencies （P〈0.05 all）;geno-type＊1/＊1 significant increase [（38.5% vs. 57.7%）,P〈0.01],＊1/＊2 significant decrease [（44.7% vs. 31.5%）,P〈0.05],and other four genotype betWeen tWo nationalities Were no significant difference （P〉0.05）;fast metabolic patter frequency （38.5% vs. 57.7%）significant increase,moderate metabolic patter frequency （52.8% vs. 31.5%）significant decrease （P〈0.01 all）in Uighur nationality patients ;In a same nationality,age had no significant impact on its genotype and allele distribution （P〉0.05 all）.Conclusion：There are significant differences in cytochrome P450 2C19 genotype and allele distribution betWeen Uighur nationality and Han nationali-ty CHD patients,Understanding those is of important significance to guide clinical medication and improve patients＆#39; prognosis.